How to Purify Your Protein With Ion Exchange Chromatography (IEX)
Does it ever feel like the hardest part of a project is just getting started on the dang thing? For lots of projects, it feels...
Articles
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What is ion exchange chromatography?
Have you ever heard the saying “opposites attract”? Well, it turns out this phrase is true in the molecular world as well. Positive and negative...
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The 2 Common Types of Ion Exchange Chromatography: Anion vs. Cation
Ion exchange chromatography includes a suite of related methods that purify proteins based on their charge. The two main types of ion exchange resins are...
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From Basic to Deadly: The Impact of pH on Protein Function and Cancer Progression
You may have heard that cancer is a genetic disease, and that is true for many types of cancers. There are many known gene mutations...
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Beyond pH 7 - Biologically Relevant pH In Diverse Environments
In Flatland, a novel by Edwin Abbott, a square narrates his flat two-dimensional world and his dreams of visiting the more limited, one-dimensional Lineland. In...
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12 Useful Detergents for Your Experiments
You might be designing a cell lysis experiment to extract your target protein in its native form, or you have isolated cell membranes from which...
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3 Common Reasons for Protein Isoelectric Point Discrepancies
Is your protein not sticking to the ion exchange column when you thought it should have? As frustrating as this result is, every experienced protein...
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How Detergents are Extremely Useful in Bioscience Experiments
Detergents are versatile chemical tools. But how exactly do they help in experiments? Some detergents break down biological membranes, and some are used to denature...
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Charged up: The Complexities and Significance of Protein Charge in Protein Purification
Do you know what really charges me up? Discussing protein electrostatics, of course! Proteins are made up of amino acids. A few amino acids are...
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What is Affinity Purification for Antibodies?
Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense when we are infected. Scientists frequently use antibodies as...
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Why Lysozyme and Zymolyase are Important in Cell Lysis Buffers
Lysozyme and zymolyase are enzymes that degrade cell walls of bacteria and yeast cells. When bacterial or yeast cells are lysed during experiments, these enzymes...
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3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto your protein of interest while cloning it...
