Fold-Assist Chemically Competent Cell User Guide
This protocol describes the use of Fold-Assist™ chaperone-competent E. coli strains to improve the folding and solubility of recombinant proteins through co-expression of molecular chaperones. It outlines procedures for transforming expression plasmids into these strains and inducing both chaperone systems and target protein expression under controlled conditions. The workflow enables rapid screening of multiple chaperone environments to identify conditions that maximize soluble protein yield.