Auxo-Agro™ Methionine Auxotrophic Agrobacterium Competent Cells

Are antibiotics hurting your plant transformants?

Agrobacterium removal is needed following a successful plant transformation. Traditionally, antibiotics are used for this purpose, but higher concentrations of antibiotics can have an adverse effect on plant tissue.

GoldBio EHA105∆Met¹ and LBA4404∆Met¹ Agrobacterium tumefaciens competent cells minimize the use of antibiotics to prevent overgrowth of the plant tissue and can help streamline your plant transformation procedures.

Benefits:

Reduces the need for antibiotics during transformation.

Increases plant transformation efficiency.

Agrobacterium can be completely killed by removing methionine from the culture media combined with a low concentration of antibiotic.

Knocking out genes to cause auxotrophy DOES NOT affect transformation capacity.¹

Prías-Blanco M, Chappell TM, Freed EF, Illa-Berenguer E, Eckert CA, Parrott WA. An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation. Transgenic Res. 202231: 661-676. doi: 10.1007/s11248-022-00328-4. PMID: 36239844.
Important note from this paper: “Switchgrass transformation was chosen to validate these strains by evaluating their performance in a difficult transformation system."

auxo agro cultures in flasks

5 free cuvette offer

Auxo-Agro® EHA105 Chemically Competent Cells

Catalog ID	Size	Pricing
CC-168-5x50	5 x 50 μL	$195.00 $156.00
CC-168-10x50	10 x 50 μL	$346.00 $276.00
CC-168-15x50	15 x 50 μL	$463.00 $370.00

Show Product Details

product description

GoldBio’s Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our EHA105 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: EHA105∆Met Format: Tubes Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

Product Specifications

Catalog ID:	CC-168
Storage/handling :	Store Competent Cells at -80°C.

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Packs & Pricing

Catalog ID	Size	Pricing
CC-168-5x50	5 x 50 μL	$195.00 $156.00
CC-168-10x50	10 x 50 μL	$346.00 $276.00
CC-168-15x50	15 x 50 μL	$463.00 $370.00

$156.00 - $370.00

Auxo-Agro® EHA105 Electrocompetent Cells

Catalog ID	Size	Pricing
CC-268-5x50	5 x 50 μL	$205.00 $164.00
CC-268-10x50	10 x 50 μL	$370.00 $296.00
CC-268-15x50	15 x 50 μL	$500.00 $400.00

Show Product Details

product description

GoldBio’s Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

GoldBio’s EHA105 Agrobacterium electrocompetent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.

Product Specifications Competent cell type: ElectroCompetent Species: A. tumefaciens Strain: EHA105∆Met Format: Tubes Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

Product Specifications

Catalog ID:	CC-268
Storage/handling :	Store Competent Cells at -80°C.

View

Packs & Pricing

Catalog ID	Size	Pricing
CC-268-5x50	5 x 50 μL	$205.00 $164.00
CC-268-10x50	10 x 50 μL	$370.00 $296.00
CC-268-15x50	15 x 50 μL	$500.00 $400.00

$164.00 - $400.00

Auxo-Agro® LBA4404 Chemically Competent Cells

Catalog ID	Size	Pricing
CC-167-5x50	5 x 50 μL	$195.00 $156.00
CC-167-10x50	10 x 50 μL	$346.00 $276.00
CC-167-15x50	15 x 50 μL	$463.00 $370.00

Show Product Details

product description

GoldBio’s new Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

Product Specifications Competent cell type: Chemical Competent Species: A. tumefaciens Strain: LBA4404∆Met Format: Tubes Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

Product Specifications

Catalog ID:	CC-167
Storage/handling :	Store Competent Cells at -80°C.

View

Packs & Pricing

Catalog ID	Size	Pricing
CC-167-5x50	5 x 50 μL	$195.00 $156.00
CC-167-10x50	10 x 50 μL	$346.00 $276.00
CC-167-15x50	15 x 50 μL	$463.00 $370.00

$156.00 - $370.00

Auxo-Agro® LBA4404 Electrocompetent Cells

Catalog ID	Size	Pricing
CC-267-5x50	5 x 50 μL	$205.00 $164.00
CC-267-10x50	10 x 50 μL	$370.00 $296.00
CC-267-15x50	15 x 50 μL	$500.00 $400.00

Show Product Details

product description

GoldBio’s Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when dual selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

Product Specifications Competent cell type: ElectroCompetent Species: A. tumefaciens Strain: LBA4404∆Met Format: Tubes Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA Blue/white screening: No

Product Specifications

Catalog ID:	CC-267
Storage/handling :	Store Competent Cells at -80°C.

View

Packs & Pricing

Catalog ID	Size	Pricing
CC-267-5x50	5 x 50 μL	$205.00 $164.00
CC-267-10x50	10 x 50 μL	$370.00 $296.00
CC-267-15x50	15 x 50 μL	$500.00 $400.00

$164.00 - $400.00