GoldBio’s Cohn Analog BSA is a highly purified, lyophilized “Fraction V” bovine serum albumin designed for sensitive applications in cell culture, microbial culture, reproductive biology, and molecular biology. Derived from the plasma of USDA-inspected healthy animals in U.S.-based facilities, this lyophilized “Fraction V” powder is produced using a proprietary, non-solvent procedure, including membrane dialysis, filtration, and a modified Cohn/heat-shock procedure, to inactivate proteases and minimize analytes.

GoldBio Cohn Analog BSA is free of carbohydrates and suitable for applications such as Western blotting, enzyme systems, protein standards, hybridizations, and protease-sensitive immunoassays. Our BSA is protease-free and low-interference, consisting of high purity with low backgrounds. Cohn Analog BSA offers a superior performance for enhancing growth and maintaining consistency in demanding workflows making it the reliable choice for both standard and sensitive molecular biology experiments.

GoldBio Caprylate Stabilized BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments. Our Caprylate Stabilized BSA is protease and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.

Sulfhydryl Blocked Bovine Serum Albumin (sBSA) is a highly purified, lyophilized albumin designed for immunoassays and enzymatic applications that require minimal reactivity. sBSA maintains a high-monomer form with reduced cross-reactivity, ensuring exceptional stability and accuracy. sBSA is ideal for applications like acridinium ester chemiluminescence and immunoassays and is compatible with maleimide conjugation for added experimental flexibility.

Sulfhydryl BSA is particularly useful in thiol-sensitive assays, capable of blocking over 90% of the free thiol groups in BSA. Sulfhydryl BSA is free from EDTA and fatty acid-stabilized, making it suitable for a wide range of assay types. This BSA is sourced from Fraction V of the original Cohn purification, ensuring reliability and consistency. In addition to its blocking capabilities, sBSA is also used in protein standards, cell culture media, and protease-sensitive immunoassays, offering versatility across various biological and biochemical applications.

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF.It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides.The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed.Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.