GoldBio CJ236 chemically competent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut- and ung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.

CJ236 chemically competent E. coli cells are free of animal-derived products and grown with animal-free media.

Kit Components

• Competent Cells
• 1 x 12 mL Recovery Media
• 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)

GoldBio CJ236 Electrocompetent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut- and ung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.

Kit Components

• Competent Cells
• 1 x 12 mL Recovery Media
• 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)

GoldBio’s GB10B-Pro™ Electrocompetent E. coli cells are equivalent to DH10B cells and are especially designed for the most demanding cloning applications. GB10B-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.

Product Specifications Competent cell type: ElectroCompetent Equivalent to: DH10B Species: E. coli Transformation efficiency:≥1 x 109 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s GB10B™ Chemically Competent E. coli cells are equivalent to DH10B cells. GB10B™ cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications Competent cell type: Chemically competent Equivalent to: DH10B Species: E. coli Transformation efficiency: ≥8.2 x 106 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s GB10B™ Electrocompetent E. coli cells are equivalent to DH10B cells. GB10B™ cells are high efficiency, ≥2 x 108 cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. GB10B™ Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications Competent cell type: ElectroCompetent Equivalent to: DH10B Species: E. coli Transformation efficiency: ≥2 x 108 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are equivalent to DH5-alpha cells and are high efficiency cells suitable for transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

GB5-alpha™ chemically competent cells are free of animal-derived products and grown with animal-free media.

GB5-alpha™ Chemically competentE. colicells are equivalent to DH5-alpha.

GoldBio’s GB5-alpha™ Electrocompetent E. coli cells are equivalent to DH5-alpha cells. GB5-alpha™ cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha™ cells due to mutations in both endA1 and recA1.

Product Specifications Competent cell type: ElectroCompetent Equivalent to: DH5-alpha Species: E. coli Transformation efficiency: ≥5 x 108 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.

Product Specifications Competent cell type: Chemically competent Derivative of: HB101 Species: E. coli Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA Blue/white screening: No

GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the lacIqZΔM15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.

Product Specifications Competent cell type: Chemically competent Derivative of: JM109 Species: E. coli Format: Tubes Transformation efficiency: ≥6 x 105 cfu/µg pUC19 DNA Blue/white screening: Yes

GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.

Product Specifications Competent cell type: Chemically competent Derivative of: RR1 Species: E. coli Format: Tubes Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA Blue/white screening: No

GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 109 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).

Product Specifications Competent cell type: ElectroCompetent Species: E. coli Format: Tubes Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA Blue/white screening: Yes