Bis-acrylamide is the most frequently used crosslinking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide powder is freely soluble in water (20 mg/mL) and 2% bis-acrylamide solution is a standard solution used in most electrophoresis applications.

Bis-acrylamide is used to create crosslinks between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Bis-acrylamide is the most frequently used cross-linking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide 2% solution is a standard solution used in most electrophoresis applications. When creating a specialized solution that differs from 2%, use bis-acrylamide powder.

Bis-acrylamide is used to create cross-links between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Tricine is a zwitterionic buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is part of the Tris family of buffers with a useful pH range of 7.4 – 8.8. Tricine is commonly used in SDS-PAGE to separate peptides that are in the molecular weight range of 1 to 100 kDa. It is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography. Tricine was determined to be the best buffer tested for use in ATP assays involving firefly luciferase. Tricine can serve as scavenger of hydroxyl radicals; this should be taken into consideration if using tricine in the presence of an enzyme with oxidase activity. Tricine forms a complex with Cu(II). The addition of Cu2+ to a solution enables tricine to be used in the Lowry protein assay. Tricine can be used as a substitute for protocols that utilize the controlled substance barbital as a buffer.

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0 and is also used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, it can also be used for many custom running and loading buffers, most often with glycine and SDS.

Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules.