Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Glycine has many applications. As an amino acid, it is found as a biological molecule in the central nervous system and has inhibitory effect, especially in the spinal cord, brainstem and retina; and is also a precursor to proteins. In eukaryotes it is an important precursor to porphyrins, and provides the C2N subunit of all purines. For the purposes of testing glycine is a significant component of some solutions used in SDS-PAGE protein analysis as a buffering agent and for removing labelling antibodies from Western Blot membranes. Glycine methyl ester is a reagent used in chemical synthesis, it can synthesize selenohydantoins when used in addition to isoselenocyanate.

Because of its charge properties and small size, glycine is commonly used as a counter-ion in SDS-PAGE applications to balance running buffer charge and electric field strength.

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0 and is also used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, it can also be used for many custom running and loading buffers, most often with glycine and SDS.

Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules.