Bis-acrylamide is the most frequently used crosslinking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide powder is freely soluble in water (20 mg/mL) and 2% bis-acrylamide solution is a standard solution used in most electrophoresis applications.

Bis-acrylamide is used to create crosslinks between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Bis-acrylamide is the most frequently used cross-linking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide 2% solution is a standard solution used in most electrophoresis applications. When creating a specialized solution that differs from 2%, use bis-acrylamide powder.

Bis-acrylamide is used to create cross-links between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Because of its charge properties and small size, glycine is commonly used as a counter-ion in SDS-PAGE applications to balance running buffer charge and electric field strength.