pSuperAgro® v4 Agrobacterium Electrocompetent Cells

pSuperAgro® v4 electrocompetent cells include helper plasmids which were designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

 


pSuperAgro® 4 Benefits:

  • Ethylene evolution suppression
  • Reduced GABA content
  • Higher callus induction frequency
  • Reduced callus browning
  • Induces higher shoot regeneration
  • Increased transient and stable transformation efficiencies

 

 

5 products

  • GV3101 (pSuperAgro® v4tet) Agrobacterium Electrocompetent Cells

    GV3101 (pSuperAgro® v4tet) Agrobacterium Electrocompetent Cells
    Catalog ID Size Pricing
    CC-614-5x50 5 x 50 μL $219.00   $175.00
    CC-614-10x50 10 x 50 μL $394.00   $315.00
    CC-614-15x50 15 x 50 μL $532.00   $425.00

    Show Product Details

    product description

    GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

    pSuperAgro v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance.

    These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

    Product Specifications

    Catalog ID: CC-614
    Storage/handling : Store Competent Cells at -80°C.
    View
    Packs & Pricing
    Catalog ID Size Pricing
    CC-614-5x50 5 x 50 μL $219.00
    $175.00
    CC-614-10x50 10 x 50 μL $394.00
    $315.00
    CC-614-15x50 15 x 50 μL $532.00
    $425.00

    $175.00 - $425.00

  • EHA105 (pSuperAgro® v4tet) Agrobacterium Electrocompetent Cells

    EHA105 (pSuperAgro® v4tet) Agrobacterium Electrocompetent Cells
    Catalog ID Size Pricing
    CC-634-5x50 5 x 50 μL $219.00   $175.00
    CC-634-10x50 10 x 50 μL $394.00   $315.00
    CC-634-15x50 15 x 50 μL $532.00   $425.00

    Show Product Details

    product description

    GoldBio’s EHA105 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

    pSuperAgro v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance

    These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

    Product Specifications

    Catalog ID: CC-634
    Storage/handling : Store Competent Cells at -80°C.
    View
    Packs & Pricing
    Catalog ID Size Pricing
    CC-634-5x50 5 x 50 μL $219.00
    $175.00
    CC-634-10x50 10 x 50 μL $394.00
    $315.00
    CC-634-15x50 15 x 50 μL $532.00
    $425.00

    $175.00 - $425.00

  • LBA4404 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells

    LBA4404 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells
    Catalog ID Size Pricing
    CC-644-5x50 5 x 50 μL $219.00   $175.00
    CC-644-10x50 10 x 50 μL $394.00   $315.00
    CC-644-15x50 15 x 50 μL $532.00   $425.00

    Show Product Details

    product description

    GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.

    pSuperAgro v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

    These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

    Product Specifications

    Catalog ID: CC-644
    Storage/handling : Store Competent Cells at -80°C.
    View
    Packs & Pricing
    Catalog ID Size Pricing
    CC-644-5x50 5 x 50 μL $219.00
    $175.00
    CC-644-10x50 10 x 50 μL $394.00
    $315.00
    CC-644-15x50 15 x 50 μL $532.00
    $425.00

    $175.00 - $425.00

  • AGL-1 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells

    AGL-1 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells
    Catalog ID Size Pricing
    CC-624-5x50 5 x 50 μL $219.00   $175.00
    CC-624-10x50 10 x 50 μL $394.00   $315.00
    CC-624-15x50 15 x 50 μL $532.00   $425.00

    Show Product Details

    product description

    GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

    v4 was designed to include AcdS and GabT activity, driven by a single promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

    These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

    Product Specifications

    Catalog ID: CC-624
    Storage/handling : Store Competent Cells at -80°C.
    View
    Packs & Pricing
    Catalog ID Size Pricing
    CC-624-5x50 5 x 50 μL $219.00
    $175.00
    CC-624-10x50 10 x 50 μL $394.00
    $315.00
    CC-624-15x50 15 x 50 μL $532.00
    $425.00

    $175.00 - $425.00

  • C58C1 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells

    C58C1 (pSuperAgro® v4) Agrobacterium Electrocompetent Cells
    Catalog ID Size Pricing
    CC-654-5x50 5 x 50 μL $219.00   $175.00
    CC-654-10x50 10 x 50 μL $394.00   $315.00
    CC-654-15x50 15 x 50 μL $532.00   $425.00

    Show Product Details

    product description

    GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

    pSuperAgro v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

    These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

    Product Specifications

    Catalog ID: CC-654
    Storage/handling : Store Competent Cells at -80°C.
    View
    Packs & Pricing
    Catalog ID Size Pricing
    CC-654-5x50 5 x 50 μL $219.00
    $175.00
    CC-654-10x50 10 x 50 μL $394.00
    $315.00
    CC-654-15x50 15 x 50 μL $532.00
    $425.00

    $175.00 - $425.00

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