2-Naphthyl myristate is a substrate for carboxylesterase. When coupled with a diazonium salt an azo-dye is formed. It can also be detected via fluorescence analysis.

8-Hydroxyquinoline-β-D-galactopyranoside is a chromogenic substrate for β-D-galactosidase, yielding a brown precipitate upon cleavage.

8-Hydroxyquinoline-β-D-glucuronic acid is a substrate for detection of β-D-glucuronidase which yields a brown precipitate upon cleavage and is used for E. coli detection.

L-Leucine beta-naphthylamide hydrochloride is a substrate for leucine aminopeptidase and similar aminopeptidases like aminopeptidase M. It is analyzed by fluorescence analysis or coupled with a coupling reagent like p-dimethylaminocinnamaldehyde to form a colored precipitate.

L-Lysyl-L-alanine 7-amido-4-methylcoumarin is used as an AMC fluorogenic substrate.

Naphthol AS-BI N-acetyl-beta-D-glucosaminide is an enzyme substrate of beta-hexosaminidase. It has also been used as an equipment sterilization indicator.

Phenyl-alpha-D-glucopyranoside is as reagent used in chemical synthesis. TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides.

Phenyl-beta-D-glucopyranoside is a substrate for beta-glucosidase.