CHAPS, also known as ((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate, is a zwitterionic surfactant primarily used to solubilize membrane proteins and other macromolecules.

Oftentimes, mild detergents are used in cell lysis applications by interfering with membrane interactions.

NDSB-201 is a protein specific additive that can be used to help solubilize and stabilize proteins at non-denaturing concentrations while preventing protein aggregation.

NDSB-201 is a member of a group of nondetergent sulfobetaines. These zwitterionic compounds can reduce aggregation and aid in refolding and renaturing of proteins found in bacterial expression systems and in inclusion bodies. Unlike detergents such as CHAPS, NDSBs are nondenaturing, even at higher concentrations and have been shown to increase the yield efficiency of membrane and nuclear proteins, among others.