IPTG Induction Guidebook: Download for Free
Optimize Your IPTG Induction!
This free guidebook helps you dial in your IPTG concentration, temperature, and timing so you can get higher protein expression.
Included inside is basic information, optimization conditions, logs, protocols and so much more. Use the form below to get your copy.
What's Inside:
This guide pulls together the essential pieces of IPTG induction so you don't have to dig through scattered resources every time you express a new construct.
How IPTG Works – simple explanation of LacI, the lac operator, and how non-metabolizable IPTG drives expression.
Standard Induction Conditions – “hot and fast” vs. “low and slow” expression strategies with example IPTG concentrations and temperatures.
IPTG Optimization Quick Reference – how to tweak IPTG levels and temperature when your protein is insoluble or under-expressed.
IPTG FAQs – answers to common questions about OD₆₀₀, IPTG dosing, solubility, and overexpression.
Induction Experiment Log – printable log to track strain, OD₆₀₀, IPTG concentration, and outcomes for each construct.
Ready-to-Use Protocols – step-by-step induction and extraction protocol, 100 mM stock solution prep, and IPTG EZ-Pak™ instructions.
Is This Guidebook Right for You?
If you’re using IPTG to induce recombinant protein expression in E. coli, this guide is built to save you time at the bench—whether you’re planning a new construct or fixing one that keeps going into inclusion bodies.
- Grad students and postdocs troubleshooting expression and solubility
- Research scientists running protein production for downstream assays
- PIs and lab managers who want standard induction defaults for their group
- Core facility staff supporting multiple expression projects