Protocol - Rifampicin Stock Solution

Rifampicin Stock Solution Instructions

50 mg/ml Rifampicin in DMSO

1. Weigh 0.5 g of rifampicin (Rifampicin, GoldBio Catalog # R-120, [CAS 13292-46-1, mw. = 822.94 g/mol]).
2. Add 10 ml DMSO. Dissolve completely.
3. Stock may be kept at -20°C for 1 year. Rifampicin may precipitate in the freezer. Dissolve again before use.

16 mg/ml Rifampicin in Methanol

1. Weigh 0.16 g of rifampicin [Rifampicin, GoldBio Catalog # R-120, (CAS 13292-46-1, MW = 822.94 g/mol)].
2. Add 10 ml Methanol. Dissolve completely.
3. Stock may be kept at -20°C for 1 year. Rifampicin may precipitate in the freezer. Dissolve again before use.
   • Note: Rifampicin will precipitate out of solution in methanol if the concentration is above 16 mg/ml.