Description
GoldBio Proteinase K Solution 20 mg/ml provides you with a convenient, ready-to-use liquid format, eliminating the need to weigh and dissolve powdered enzyme before use.
The defined 20 mg/ml concentration simplifies dilution and addition to samples while supporting consistent preparation across experiments. This format is especially useful for laboratories performing routine nucleic acid isolation, sample digestion, and other applications requiring frequent Proteinase K use.
Proteinase K is a highly active, nonspecific serine protease that cleaves peptide bonds adjacent to aliphatic, aromatic, and hydrophobic amino acids. Its ability to digest proteins and inactivate nucleases such as DNases and RNases makes it particularly valuable for the isolation and preparation of high molecular weight DNA and RNA.
GoldBio Proteinase K undergoes two sterile filtration steps during separation and purification, followed by plate culture analysis to verify the removal of viable organisms. The combination of robust proteolytic activity and a convenient 20 mg/ml solution provides researchers with a reliable option for nucleic acid preparation, protein digestion, enzyme characterization, and other molecular biology applications.
Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.
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Applications
- Digestion of unwanted proteins
- Removal of endotoxins bound to cationic proteins such as lysozyme and RNaseA
- Removal of nucleases for in situ hybridization
- Prion research with respect to TSE (transmissible spongiform encephalopathies)
- Protease footprinting Mitochontrial isolation
- Isolation of genomic DNA
- Isolation of cytoplasmic RNA
- Isolation of highly native DNA or RNA
Storage/Handling
Store at -20°C.
Reconstitute in 50mM Tris-HCl (pH 8.0), 3mM CaCl2.
Proteinase K Quick Answers:
How do you inactivate proteinase K?
Inactivate proteinase K by heating it to 95˚C for 10 minutes. You can also permanently inactivate proteinase K by using protease inhibitors such as PMSF and AEBSF (Pefabloc®).
What is the best temperature for proteinase K activation?
The best temperature for proteinase K activity is between 50-65˚C. Higher temperatures help with protein unfolding, which makes it easier for proteinase K to break down proteins.
How do you store proteinase K?
Aliquot proteinase K stock solution and store at -20˚C for up to 1 year.
Store lyophilized proteinase K powder desiccated at -20˚C for up to 2 years.
How do you dissolve proteinase K?
Proteinase K is water soluble and it can also be dissolved in Tris or PBS. Dissolving proteinase K with PBS is a little challenging, which may be due to pH. It's best to add proteinase K a little bit at a time while mixing into solution.
What is the optimal pH when working with proteinase K?
The optimal pH when working with proteinase K is between 7.5 - 12.0
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