EvaGreen® Dye, 20x in Water

Product Description

EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, making it a great alternative to SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et al. (2001). Mutation Research, 492(1), 91-97).

The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

Features

  • Low PCR inhibition
Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology

  • Highly Sensitive
Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals

  • Nonmutagenic and noncytotoxic
Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes

  • Compatible with fast PCR protocols
Minimal interference to PCR makes it possible to significantly shorten the chain extension time

  • Compatible with multiplex PCR
No dye migration from amplicon to amplicon when used at the recommended concentration

  • Unsurpassed thermal stability, chemical stability and photostability
No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
  • Spectrally similar to SYBR® Green I
Compatible with all major brand qPCR instruments and enzyme systems

Storage/Handling: Store at 4°C. Protect from light.

Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

EvaGreen® dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.

Product Specifications

Catalog ID E-670
Storage/Handling Store at 4°C. Protect from light.

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