Acriflavine HCl is a compound predominantly used in cellular biology for fluorescence staining. It intercalates between the base pairs of DNA and RNA. Among the acridine dyes, acriflavine has the highest affinity for DNA. Acriflavine stains eukaryotic cells exceptionally well in humic substance environments. It provides a robust experimental consistency and reduces the effect of minor experimental errors. The binding of acriflavine to DNA can be observed from a characteristic red shift in an absorption spectrum. While having a high affinity for DNA, acriflavine is limited to euchromatin for both DNA and RNA.
Acriflavine has also been found to inhibit heterodimeric transcription factors, specifically called hypoxia-inducible factors (HIFs). Studying the hyperactivity of HIFs has become a popular in oncology research, where chemicals like acriflavine are used to disrupt HIF activity of malignant cells. HIFs are activated in hypoxic conditions and promote angiogenesis to alleviate stress. They are normally regulated through proline and asparagine hydroxylation. Acriflavine halts HIF activity, specifically by binding to a subdomain of HIF-1α and HIF-2α.
MW: 468.98 g/mol
Storage/Handling: Store at 4°C.
PubChem Chemical ID: 443101
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|Storage/Handling||Store at 4°C.|