GB10B™ Chemically Competent E. coli Cells

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Description

GoldBio’s GB10B™ Chemically Competent E. coli cells are equivalent to DH10B cells. GB10B™ cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

GB10B™ Chemically competent E. coli cells are equivalent to DH10B.

Reagents Needed for One Reaction

  • GB10B™ chemically competent cells: 50 µL
  • DNA (or pUC19 Control, 500 pg/µL): 2 µL
  • Recovery medium: 1 mL


Kit Components

  • Competent Cells
  • 1 x 12 mL Recovery Media
  • 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)

Storage/Handling

This product may be shipped on dry ice. GB10B™ Chemically Competent E. coli cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

Genomic Features

  • Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
  • mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine

Genotype

F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥8.2 x 106 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Calculation of Transformation Efficiency

Transformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1µg of plasmid into a given volume of competent cells.

  • TE = Colonies/µg/Dilution
    • Colonies = the number of colonies counted
    • µg = amount of DNA transformed in µg
    • Dilution = total dilution of the DNA before plating
    • Example: Transform 1 µL of (10 pg/µL) control plasmid into 25 µL of cells, add 975 µL of Recovery Medium. Dilute 10 µL of this in 990 µL of Recovery Medium and plate 50 µL. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:

    Colonies = 250
    µg of DNA = 0.00001
    Dilution = 10/1000 x 50/1000 = 0.0005
    TE = 250/0.00001/0.0005 = 5.0 × 1010

pUC Plasmid Vector

GB10B™ Chemically Competent E. coli Cells

View Sizes & Pricing

Catalog Number:
CC-100-5x50
CAS Number:
$61.00 $48.00

Availability:
In Stock
Shipping:
Shipping calculated at checkout

    Description

    GoldBio’s GB10B™ Chemically Competent E. coli cells are equivalent to DH10B cells. GB10B™ cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

    GB10B™ Chemically competent E. coli cells are equivalent to DH10B.

    Reagents Needed for One Reaction

    • GB10B™ chemically competent cells: 50 µL
    • DNA (or pUC19 Control, 500 pg/µL): 2 µL
    • Recovery medium: 1 mL


    Kit Components

    • Competent Cells
    • 1 x 12 mL Recovery Media
    • 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)

    Storage/Handling

    This product may be shipped on dry ice. GB10B™ Chemically Competent E. coli cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

    Genomic Features

    • Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
    • mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine

    Genotype

    F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-

    Quality Control

    Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥8.2 x 106 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

    General Guidelines

    • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
    • Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

    Calculation of Transformation Efficiency

    Transformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1µg of plasmid into a given volume of competent cells.

    • TE = Colonies/µg/Dilution
      • Colonies = the number of colonies counted
      • µg = amount of DNA transformed in µg
      • Dilution = total dilution of the DNA before plating
      • Example: Transform 1 µL of (10 pg/µL) control plasmid into 25 µL of cells, add 975 µL of Recovery Medium. Dilute 10 µL of this in 990 µL of Recovery Medium and plate 50 µL. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:

      Colonies = 250
      µg of DNA = 0.00001
      Dilution = 10/1000 x 50/1000 = 0.0005
      TE = 250/0.00001/0.0005 = 5.0 × 1010

    pUC Plasmid Vector

    Product Specifications

    Catalog ID: CC-100
    Storage/handling: Store Competent Cells at -80°C.

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