EHA105 Agrobacterium ElectroCompetent Cells

Product Description

GoldBio’s EHA105 Agrobacterium ElectroCompetent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

Test-drive GoldBio’s competent cells with our trial sizes . Each trial size is available in 3 x 50 μl volumes. Look for the TR mark in the catalog number for trial size packs.

Table 1: Antibiotic selection for GoldBio’s Agrobacterium strains

Antibiotic Selection

Amp

Carb

Chlor

Gent

Kan

Rif

Spect

Strep

Tet

100
µg/ml

100
µg/ml

30
µg/ml

100
µg/ml

30
µg/ml

50
µg/ml

25
µg/ml

50
µg/ml

50
µg/ml

50
µg/ml

GV3101

S

S

R

PR

R

S

R

PR

R

S

EHA 105

S

S

R

R

R

S

R

R

R

S

LBA 4404

S

S

S

n/a

S

S

R

S

R

S

AGL-1

PS

R

S

n/a

S

S

R

S

R

S

C58C1

S

S

S

n/a

S

S

R

S

R

S

S = Sensitive
R = Resistant
PS = Partial Sensitive (Some growth, but no colonies)
PR = Partial Resistance (Small colonies or some growth in concentrated areas)


Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

Storage/Handling: This product may be shipped on dry ice. EHA105 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

Genomic Features

  • ≥1 x 107 cfu/µg efficiency with electroporation.

Reagents Needed for One Reaction

  • EHA105 ElectroCompetent Agrobacterium: 25 µl
  • DNA (pCAMBIA1391z, 500 pg/µl): 1 µl
  • Recovery medium: 1 ml

Quality Control

Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

  • Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
  • Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Calculation of Transformation Efficiency

Transformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 µg of plasmid into a given volume of competent cells.

  • TE = Colonies/µg/Dilution
    • Colonies = the number of colonies counted
    • µg = amount of DNA transformed in µg
    • Dilution = total dilution of the DNA before plating
Example: Transform 1 µl of (10 pg/µl) control plasmid into 25 µl of cells, add 975 µl of Recovery Medium. Dilute 10 µl of this in 990 µl of Recovery Medium and plate 50 µl. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:

Colonies = 250
µg of DNA = 0.00001
Dilution = 10/1000 x 50/1000 = 0.0005
TE = 250/0.00001/0.0005 = 5.0 × 1010

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