Protein A Agarose Resin (Pharmaceutical grade for research)
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk 5 or 25 ml quantities or in Prepacked 1 and 5 ml columns for immediate gravity flow purifications.
Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).
Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).
Protein A is temperature stable and it retains its native conformation even in the presence of denaturing agents.
Storage/Handling: Store at 4°C. Do not freeze.
|Storage/Handling||store at 4°C. Do NOT freeze.|
Comparison of Relative Affinity of Protein A and Protein G for various polyclonal and monoclonal IgG antibodies by Harlow and Lane (1988)
Protein A agarose resin column - Antibody binding protocol for a low pressure gravity column system.
Immunoprecipitation protocol utilizing agarose resins.