Spectinomycin Dihydrochloride Pentahydrate, USP Grade
Spectinomycin is an aminocyclitol antibiotic isolated from Streptomyces spectabilis. It is effective against gram-positive and gram-negative bacteria, but most commonly used against Gonococcus species. It has also been used to monitor cell fate during leaf development and as a selection marker in plants to display successful transformation of the Spcr gene.
Spectinomycin functions by binding the 30s ribosomal subunit, inhibiting tRNA translation and protein synthesis. Resistance to this antibiotic is conferred through the aadA gene and mutated rpsE gene.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.
Spectinomycin Dihydrochloride Pentahydrate
Formula: C14H24N2O7 · 2HCl · 5H2O
MW: 495.35 g/mol
Storage/Handling: Store desiccated at -20°C. Soluble in water.
PubChem Chemical ID: 30971
|Storage/Handling||Store desiccated at -20°C.|
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Tobacco Plasmid Transformation using Spectinomycin, by Khan and Maliga (1999)