Tetracycline is a broad-spectrum antibiotic targeting gram-negative and gram-positive bacteria. It also displays toxicity against certain eukaryotic cells. Tetracycline is used in multidrug resistance studies and in cell culture applications as a selective agent. Additionally, it promotes expression of the P450 proteins.
Tetracyclines are antibiotics made up of a four-ring system. They bind the 30s ribosomal subunit, preventing the aminoacyl-tRNA from attaching to the A site. Consequently, protein synthesis is inhibited. Resistance to tetracycline arises from loss of cell wall permeability and the tetR gene.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.
MOLECULAR BIOLOGY GRADE
Formula: C 22H24N2O8HCl
MW: 480.9 g/mol
Storage/Handling: Store desiccated at -20°C, and protect from light. Soluble in water.
PubChem Chemical ID: 5359252
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at -20°C. Protect from light.|
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample Certificate of Analysis - Tetracycline Hydrochloride
COA for example only. Actual tests may differ.