Vancomycin Hydrochloride, USP Grade

Description

Vancomycin Hydrochloride is a high-purity glycopeptide antibiotic trusted by researchers for its precision and potency in studying Gram-positive bacterial inhibition. With ≥900 µg/mg potency and USP-certified purity, this compound is ideal for applications including MIC profiling, resistance modeling in MRSA and VRE, nanoparticle-based delivery research, and biofilm penetration assays.

Its compatibility with liposomal and silica-based nanocarriers enables controlled release and enhanced efficacy in both in vitro and in vivo systems. Researchers can rely on GoldBio’s USP-grade vancomycin for robust data, reproducibility, and superior performance across antimicrobial resistance and drug delivery studies.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Key Specifications & Identity

Property	Value / Description
Synonyms / Alternate Names	Vancomycin HCl; Vancomycin Hydrochloride
CAS Number	1404-93-9
PubChem CID	14970
Molecular Formula	C₆₆H₇₅Cl₂N₉O₂₄ · HCl (salt form)
Molecular Weight	1,485.71 g/mol (including HCl)
Potency	> 1,000 µg vancomycin equivalent per mg (on anhydrous basis)
Solubility	Soluble in water
Storage & Handling	Store desiccated at –20 °C, protected from moisture and light

Mode of Action & Biological Relevance

Vancomycin is an antibiotic whose primary mode of action is inhibition of bacterial cell wall synthesis. Specifically, it binds to the D-Ala–D-Ala terminal residues of peptidoglycan precursors, preventing polymerization and cross-linking.
Because it targets Gram-positive cell wall pathways, vancomycin is particularly effective against Gram-positive bacteria (e.g. Staphylococcus spp., Enterococcus spp.).
In research settings, it is often used at selective concentrations to suppress unwanted Gram-positive bacterial growth in mixed cultures or to apply selective pressure in antibiotic resistance studies.

Suggested Applications & Usage Notes

Selective media / contamination control
Incorporate vancomycin into culture media (bacterial, plant, mammalian) to suppress Gram-positive contaminants while allowing growth of desired organisms.
Antibiotic resistance / susceptibility assays
Use as a reference antibiotic in MIC (minimum inhibitory concentration) testing, resistance screening, or mechanistic studies.
Combination / synergy experiments
Combine with other antibiotics (e.g. β-lactams, aminoglycosides) to investigate synergistic or antagonistic interactions in bacterial systems.
Modeling resistance evolution / genetic studies
Apply vancomycin stress in evolutionary experiments or to select for resistant mutants for downstream genomic/phenotypic analysis.

Common Research Applications:

(Click each for more information)

Antibiotic Susceptibility Testing and MIC Profiling

Purpose: Used as a gold-standard glycopeptide for determining MIC values in Gram-positive pathogens such as Staphylococcus aureus and Enterococcus spp.
How It Works: Vancomycin inhibits peptidoglycan synthesis by binding the D-Ala-D-Ala moiety, preventing bacterial cell wall crosslinking. MIC profiling determines the lowest concentration inhibiting visible growth.
Applications: Clinical microbiology research, MIC creep surveillance, and benchmarking resistance in MRSA and VRE strains.

van Hal, S. J., Lodise, T. P., & Paterson, D. L. (2012). The clinical significance of vancomycin minimum inhibitory concentration in Staphylococcus aureus infections: A systematic review and meta-analysis. Clinical Infectious Diseases, 54(6), 755–771.

Modeling Resistance Mechanisms in Gram-Positive Pathogens

Purpose: Used to induce, track, and characterize vancomycin resistance phenotypes such as VISA and VRE.
How It Works: Exposure of bacteria to vancomycin in vitro facilitates selection of strains with cell wall thickening, altered targets (D-Ala-D-Lac), or horizontal gene transfer mechanisms such as the vanA operon.
Applications: Genetic and phenotypic resistance analysis, molecular pathway studies, and mechanism-of-action research.

Arthur, M., & Courvalin, P. (1993). Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrobial Agents and Chemotherapy, 37(8), 1563–1571.

Nanoparticle and Liposomal Drug Delivery Systems

Purpose: Improve vancomycin bioavailability, intracellular delivery, and pharmacokinetics using encapsulation in nanocarriers.
How It Works: Vancomycin is encapsulated in liposomes, PLGA nanoparticles, or related delivery systems to improve tissue penetration and macrophage uptake.
Applications: MRSA infection modeling, controlled-release testing, and delivery optimization in host–pathogen systems.

Sande, L., et al. (2012). Liposomal encapsulation of vancomycin improves killing of methicillin-resistant Staphylococcus aureus in a murine infection model. Journal of Antimicrobial Chemotherapy, 67(9), 2191–2194.

Biofilm Penetration and Disruption Studies

Purpose: Evaluate vancomycin’s ability to inhibit and disrupt bacterial biofilms, especially when delivered via advanced materials.
How It Works: Mesoporous silica nanoparticles and similar carriers improve delivery of vancomycin to embedded bacterial cells within biofilms.
Applications: MRSA biofilm inhibition, implant surface coatings, and chronic infection models.

Memar, M. Y., et al. (2023). Antibacterial and biofilm-inhibitory effects of vancomycin-loaded mesoporous silica nanoparticles on methicillin-resistant Staphylococcus aureus. Archives of Microbiology, 205(1), 109.

Selective Media for Isolation of Gram-Negative Bacteria

Purpose: Suppress Gram-positive bacteria to enable isolation of Gram-negative strains in complex environmental or clinical samples.
How It Works: Vancomycin is incorporated into selective media to inhibit Gram-positive growth while allowing resistant Gram-negative organisms such as carbapenem-resistant Enterobacteriaceae (CRE) to grow.
Applications: Environmental sampling, hospital-acquired infection surveillance, and food safety testing.

Nordmann, P., Poirel, L., & Dortet, L. (2012). Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerging Infectious Diseases, 18(9), 1503–1507.

Usage Tips:

Always validate effective concentration for your specific organism/culture system; vancomycin’s effective range depends on strain sensitivity.
Solubilize fresh stocks in sterile water; filter-sterilize if necessary.
Use matched vehicle or salt controls to account for ionic / osmotic effects.
Refer to the Certificate of Analysis (COA) to confirm potency and lot-specific data before use.

Safety & Disclaimer

For research use only. Not for human or veterinary use.
Vancomycin can be hazardous; handle in compliance with institutional safety protocols (PPE, containment, fume hood).
Avoid inhalation, ingestion, or contact with skin/eyes.
Dispose of waste and unused material in accordance with institutional and regulatory guidelines for antibiotics and chemical agents.

Storage/Handling:

Store desiccated at -20°C. Soluble in water.

Vancomycin Hydrochloride, USP Grade

Catalog Number:

V-200-1

CAS Number:

1404-93-9

Price:

$71.00 $63.00

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Description

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.

Key Specifications & Identity

Property	Value / Description
Synonyms / Alternate Names	Vancomycin HCl; Vancomycin Hydrochloride
CAS Number	1404-93-9
PubChem CID	14970
Molecular Formula	C₆₆H₇₅Cl₂N₉O₂₄ · HCl (salt form)
Molecular Weight	1,485.71 g/mol (including HCl)
Potency	> 1,000 µg vancomycin equivalent per mg (on anhydrous basis)
Solubility	Soluble in water
Storage & Handling	Store desiccated at –20 °C, protected from moisture and light

Mode of Action & Biological Relevance

Vancomycin is an antibiotic whose primary mode of action is inhibition of bacterial cell wall synthesis. Specifically, it binds to the D-Ala–D-Ala terminal residues of peptidoglycan precursors, preventing polymerization and cross-linking.
Because it targets Gram-positive cell wall pathways, vancomycin is particularly effective against Gram-positive bacteria (e.g. Staphylococcus spp., Enterococcus spp.).
In research settings, it is often used at selective concentrations to suppress unwanted Gram-positive bacterial growth in mixed cultures or to apply selective pressure in antibiotic resistance studies.

Suggested Applications & Usage Notes

Selective media / contamination control
Incorporate vancomycin into culture media (bacterial, plant, mammalian) to suppress Gram-positive contaminants while allowing growth of desired organisms.
Antibiotic resistance / susceptibility assays
Use as a reference antibiotic in MIC (minimum inhibitory concentration) testing, resistance screening, or mechanistic studies.
Combination / synergy experiments
Combine with other antibiotics (e.g. β-lactams, aminoglycosides) to investigate synergistic or antagonistic interactions in bacterial systems.
Modeling resistance evolution / genetic studies
Apply vancomycin stress in evolutionary experiments or to select for resistant mutants for downstream genomic/phenotypic analysis.

Common Research Applications:

(Click each for more information)

Antibiotic Susceptibility Testing and MIC Profiling

Purpose: Used as a gold-standard glycopeptide for determining MIC values in Gram-positive pathogens such as Staphylococcus aureus and Enterococcus spp.
How It Works: Vancomycin inhibits peptidoglycan synthesis by binding the D-Ala-D-Ala moiety, preventing bacterial cell wall crosslinking. MIC profiling determines the lowest concentration inhibiting visible growth.
Applications: Clinical microbiology research, MIC creep surveillance, and benchmarking resistance in MRSA and VRE strains.

Modeling Resistance Mechanisms in Gram-Positive Pathogens

Purpose: Used to induce, track, and characterize vancomycin resistance phenotypes such as VISA and VRE.
How It Works: Exposure of bacteria to vancomycin in vitro facilitates selection of strains with cell wall thickening, altered targets (D-Ala-D-Lac), or horizontal gene transfer mechanisms such as the vanA operon.
Applications: Genetic and phenotypic resistance analysis, molecular pathway studies, and mechanism-of-action research.

Arthur, M., & Courvalin, P. (1993). Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrobial Agents and Chemotherapy, 37(8), 1563–1571.

Nanoparticle and Liposomal Drug Delivery Systems

Purpose: Improve vancomycin bioavailability, intracellular delivery, and pharmacokinetics using encapsulation in nanocarriers.
How It Works: Vancomycin is encapsulated in liposomes, PLGA nanoparticles, or related delivery systems to improve tissue penetration and macrophage uptake.
Applications: MRSA infection modeling, controlled-release testing, and delivery optimization in host–pathogen systems.

Biofilm Penetration and Disruption Studies

Purpose: Evaluate vancomycin’s ability to inhibit and disrupt bacterial biofilms, especially when delivered via advanced materials.
How It Works: Mesoporous silica nanoparticles and similar carriers improve delivery of vancomycin to embedded bacterial cells within biofilms.
Applications: MRSA biofilm inhibition, implant surface coatings, and chronic infection models.

Selective Media for Isolation of Gram-Negative Bacteria

Purpose: Suppress Gram-positive bacteria to enable isolation of Gram-negative strains in complex environmental or clinical samples.
How It Works: Vancomycin is incorporated into selective media to inhibit Gram-positive growth while allowing resistant Gram-negative organisms such as carbapenem-resistant Enterobacteriaceae (CRE) to grow.
Applications: Environmental sampling, hospital-acquired infection surveillance, and food safety testing.

Nordmann, P., Poirel, L., & Dortet, L. (2012). Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerging Infectious Diseases, 18(9), 1503–1507.

Usage Tips:

Always validate effective concentration for your specific organism/culture system; vancomycin’s effective range depends on strain sensitivity.
Solubilize fresh stocks in sterile water; filter-sterilize if necessary.
Use matched vehicle or salt controls to account for ionic / osmotic effects.
Refer to the Certificate of Analysis (COA) to confirm potency and lot-specific data before use.

Safety & Disclaimer

For research use only. Not for human or veterinary use.
Vancomycin can be hazardous; handle in compliance with institutional safety protocols (PPE, containment, fume hood).
Avoid inhalation, ingestion, or contact with skin/eyes.
Dispose of waste and unused material in accordance with institutional and regulatory guidelines for antibiotics and chemical agents.

Storage/Handling:

Store desiccated at -20°C. Soluble in water.

Catalog ID:	V-200
CAS #:	1404-93-9
MW:	1485.71 g/mol
Storage/handling:	Store desiccated at -20°C.
PubChem Chemical ID:	6420023
IUPAC Name:	(1S,2R,18R,19R,22S,25R,28R,40S)-48-[(2S,3R,4S,5S,6R)-3-[(2S,4S,5S,6S)-4-amino-5-hydroxy-4,6-dimethyloxan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-22-(2-amino-2-oxoethyl)-5,15-dichloro-2,18,32,35,37-pentahydroxy-19-[[(2R)-4-methyl-2-(methylamino)pentanoyl]amino]-20,23,26,42,44-pentaoxo-7,13-dioxa-21,24,27,41,43-pentazaoctacyclo[26.14.2.23,6.214,17.18,12.129,33.010,25.034,39]pentaconta-3,5,8(48),9,11,14,16,29(45),30,32,34(39),35,37,46,49-pentadecaene-40-carboxylic acid;hydrochloride
InChI:	InChI=1S/C66H75Cl2N9O24.ClH/c1-23(2)12-34(71-5)58(88)76-49-51(83)26-7-10-38(32(67)14-26)97-40-16-28-17-41(55(40)101-65-56(54(86)53(85)42(22-78)99-65)100-44-21-66(4,70)57(87)24(3)96-44)98-39-11-8-27(15-33(39)68)52(84)50-63(93)75-48(64(94)95)31-18-29(79)19-37(81)45(31)30-13-25(6-9-36(30)80)46(60(90)77-50)74-61(91)47(28)73-59(89)35(20-43(69)82)72-62(49)92;/h6-11,13-19,23-24,34-35,42,44,46-54,56-57,65,71,78-81,83-87H,12,20-22,70H2,1-5H3,(H2,69,82)(H,72,92)(H,73,89)(H,74,91)(H,75,93)(H,76,88)(H,77,90)(H,94,95);1H/t24-,34+,35-,42+,44-,46+,47+,48-,49+,50-,51+,52+,53+,54-,56+,57+,65-,66-;/m0./s1
InChIKey:	LCTORFDMHNKUSG-XTTLPDOESA-N
SMILES:	CC1C(C(CC(O1)OC2C(C(C(OC2OC3=C4C=C5C=C3OC6=C(C=C(C=C6)C(C(C(=O)NC(C(=O)NC5C(=O)NC7C8=CC(=C(C=C8)O)C9=C(C=C(C=C9O)O)C(NC(=O)C(C(C1=CC(=C(O4)C=C1)Cl)O)NC7=O)C(=O)O)CC(=O)N)NC(=O)C(CC(C)C)NC)O)Cl)CO)O)O)(C)N)O.Cl

Vancomycin Hydrochloride, USP Grade

Vancomycin Hydrochloride, USP Grade

View Sizes & Pricing

Product Specifications

Technical Documentation

SDS (MSDS) V-200

V-200 Specification Sheet

Antibiotic Testing Procedure

Transformed Plant Morphology Examination after Antibiotic Selection Protocol

Vancomycin Stock Solution

GoldBio Disk Diffusion Assay Protocol

CITATIONS