X-α-Gal is a chromogenic substrate for α-galactosidase, yielding a blue precipitate. X-α-Gal is used for detecting two-hybrid interactions directly on the culture media. This substrate eliminates the need for β-galactosidase liquid and filter-lift assays in two-hybrid analysis.
X-α-Gal is commonly used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacilli species.
MOLECULAR BIOLOGY GRADE
MW: 408.64 g/mol
PubChem Chemical ID: 10173103
Storage/Handling:Store at -20°C. Protect from light.
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store at -20°C. Protect from light.|
A protocol for the use of X-alpha Gal for blue-white screening of yeast or bacterial colonies containing alpha-galactosidase.
Sample Certificate of Analysis - X-α-gal
COA for example only. Actual tests may differ.