X-gluc (CHX salt)
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
(X-glu, X-glcA, BCIG)
5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt
MOLECULAR BIOLOGY GRADE
Formula: C14H13BrClNO7 • C6H13N
MW: 521.79 g/mol
Storage/Handling: Store desiccated at -20°C. Protect from light.
Soluble in DMF.
Purity: greater than 99% (HPLC)
Thin Layer Chromatogram: single spot
Biological Activity: indigo-blue assay
Elemental Analysis: Agrees with theoretical values
PubChem Chemical ID: 16760327
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at -20°C. Protect from light.|
Pollen Staining for GUS Activity - adapted from a protocol by Dr. Daphne Preuss.
Procedures for GUS gene expression, fluorimetric and fluorigenic assays by Dr. Paul Bottino, Plant Molecular Genetics (retired), University of Maryland.