Description GoldBio’s Ampicillin (Sodium) EZ Pak™ for 100 mg/mL Solution is a complete, ready-to-use antibiotic preparation kit designed to eliminate the time, variability, and mess associated with traditional antibiotic stock preparation.
Each EZ Pak™ includes pre-weighed ampicillin sodium powder, a sterile filter, and a sterile container, allowing researchers to generate a sterile, accurately concentrated antibiotic solution in minutes, without weighing, calculations, or cleanup.
The EZ Pak™ format ensures consistent 100 mg/mL stock solutions every time, making it ideal for busy research labs, shared facilities, tissue culture workflows, and teaching environments where speed, consistency, and sterility are critical.
Ampicillin is a widely used β-lactam antibiotic for bacterial selection in molecular cloning and protein expression. It is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. Ampicillin is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella . By inhibiting bacterial cell wall synthesis, it enables selective growth of plasmid-containing bacteria carrying ampicillin resistance genes. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.
The Ampicillin (Sodium) EZ Pak™ is the fastest and easiest way to make a set amount of sterile ampicillin (sodium) solution. The kit includes pre-weighed ampicillin (sodium) powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2 O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio ampicillin (sodium) and the sterile solution is ready for tissue culture, bacterial media, or other appropriate use.
The 25 ml, 50 ml, and 100 ml require a vacuum manifold for easy vacuum filtering, while 10 ml pack size comes with a syringe filter.
Key Benefits
No Weighing or Measuring Required : Pre-weighed antibiotic eliminates balance use and calculation errors
Fast, Simple Preparation : Add sterile water, filter, and the solution is ready to use
Consistent Concentration Every Time : Reliable 100 mg/mL stock improves reproducibility
Sterile by Design : Built-in filtration supports contamination-sensitive workflows
Kit components
Pre-weighed Ampicillin (Sodium) powder
Sterile filter
Syringe filter (10 mL pack size)
Vacuum filter (25 mL, 50 mL, and 100 mL pack sizes; requires vacuum manifold)
Sterile container for the filtered antibiotic solution
Common Applications
· Used for the preparation of sterile ampicillin stock solutions for routine laboratory use
· Antibiotic selection of ampicillin-resistant bacteria in cloning and plasmid maintenance workflows
· Supplementation of bacterial growth media (liquid cultures and agar plates)
· Molecular cloning and recombinant protein expression experiments
· Transformation validation and plasmid retention in E. coli
Storage/Handling
Store desiccated at -20°C.
How to Prepare a Sterile Antibiotic Solution Using an EZ Pak™
VIDEO