Carbenicillin (Disodium) EZ Pak™ for 100 mg/mL Solution
Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species. It can be used in selection protocols and is often preferred over ampicillin due to its increased stability. This allows for less contamination of unwanted bacterial colonies. In addition, carbenicillin has been used to regulate bacterial growth in plants, yielding low regeneration frequencies. It can be used in genetic transformation applications to select for AmpR transformed cells.
Penicillins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a five-membered thiazolidine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.
The Carbenicillin (disodium) EZ PakTM is the fastest and easiest way to make a set amount of sterile carbenicillin (disodium) solution. The kit includes pre-weighed carbenicillin (disodium) powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ PakTM includes high quality GoldBio carbenicillin (disodium) and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.
The 25 ml, 50 ml, and 100 ml requires a vacuum manifold for easy vacuum filtering while the 10 ml pack size comes with syringe filter.
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Examine the Effect of Carbenicilln, Cefotaxime and Vancomycin on Plant Morphogenesis by Teixeira da Silva, JA and S. Fukai (2001)