Description
Tumor necrosis factor (or TNF-α), also called cachectin, is a potent pro-inflammatory cytokine widely used to investigate immune signaling, inflammation, cell survival, and programmed cell death.
Biologically active TNF-α forms homotrimers and signals primarily through the TNF receptors TNFR1 (p55) and TNFR2 (p75), initiating downstream pathways that can regulate NF-κB activation, inflammatory gene expression, proliferation, and apoptosis.
This untagged recombinant form is well suited for experiments requiring biologically active human TNF-α without an affinity tag.
Produced in E. coli and purified to greater than 98%, it can be used for cytokine stimulation, TNF receptor signaling, cytotoxicity assays, immune cell research, and studies of inflammatory responses.
Formulation:
Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.
Sequence:
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Storage/Handling:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum.
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Human Tumor Necrosis Factor
TNF-α, TNF-alpha
Cachectin, Cachexin
MW: ~17.5 kDa (158 amino acid)
Purity: >98% by SDS-PAGE and HPLC analyses
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml in the presence of actinomycin D.
Accession Number: P01375
HGNC ID: 11892
Source: Escherichia coli
Additional resources for this product may be found at:
OMIM
NOT FOR HUMAN USE!
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