G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.
G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.
This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.
Formula: C20H40N4O10 · 2H2SO4
MW: 692.71 g/mol
Storage/Handling: Store desiccated at -20°C.
PubChem Chemical ID: 16760463
Developing a Titration Kill Curve for cell cultures with G418, Hygromycin and Puromycin
BaF3 cell culture and Mitogenic assay for FGFR expressing BAF3 cells: by Dave Ornitz, Ornitz Lab. Washington University, St.Louis, MO
G418 culture and plate preparation protocol for bacteria, plant and mammalian cells and yeast.
Protocol for yeast plate preparation and screening of transformed yeast.