G418 Sulfate

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Description

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.

G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.

This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.

Product Specifications:
G418;
Geneticin

Formula: C20H40N4O10 · 2H2SO4

MW: 692.71 g/mol

Storage/Handling: Store desiccated at -20°C.

PubChem Chemical ID: 16760463

G418 Sulfate

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Catalog Number:
G-418-1
CAS Number:
108321-42-2
$50.00

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    Description

    G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.

    G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.

    This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.

    Product Specifications:
    G418;
    Geneticin

    Formula: C20H40N4O10 · 2H2SO4

    MW: 692.71 g/mol

    Storage/Handling: Store desiccated at -20°C.

    PubChem Chemical ID: 16760463

    Product Specifications

    Catalog ID: G-418
    CAS #: 108321-42-2
    MW: 692.71 g/mol
    Storage/handling: Store desiccated at -20°C.

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