• object(anvilstl\commercio\models\Category)#413 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(27) ["root"]=> int(1) ["lft"]=> int(166) ["rgt"]=> int(167) ["level"]=> int(3) ["name"]=> string(26) "Dyes, Stains, & Indicators" ["url_name"]=> string(22) "dyes-stains-indicators" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(531) "Gold Biotechnology's dyes and stains are used in numerous biological applications, including protein staining, electrophoresis, bacterial classification and Western staining. GoldBio’s catalog of high-quality dyes include Basic Fuschin, Brilliant Green, Bromocresol Green, Bromophenol Blue, Coomassie Stains, Crystal Violet, Orange G, Ponceau S and Xylenol Blue, among others. GoldBio guarantees high-quality products with excellent quality control and at a price that means you can focus your research money on your research." ["hide"]=> int(0) ["third_party_id"]=> int(226) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(140) "GoldBio’s selection of dyes and stains include Basic Fuschin, Brilliant Green, Coomassie Stains and more, ideal for a variety of research." 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GoldBio guarantees high-quality products with excellent quality control and at a price that means you can focus your research money on your research." ["hide"]=> int(0) ["third_party_id"]=> int(226) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(140) "GoldBio’s selection of dyes and stains include Basic Fuschin, Brilliant Green, Coomassie Stains and more, ideal for a variety of research." 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  • object(anvilstl\commercio\models\Category)#440 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(125) ["root"]=> int(393) ["lft"]=> int(36) ["rgt"]=> int(37) ["level"]=> int(1) ["name"]=> string(22) "Coomassie Stains (old)" ["url_name"]=> string(16) "coomassie-stains" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(825) "

    Coomassie stains are a popular method of protein gel electrophoresis or SDS-PAGE staining. Gold Biotechnology has both G250 (blue) and R250 (red) dyes available for purchase. These dyes bind to proteins to form a protein-dye complex while the polyacrylamide gels can be easily destained with a mixture of acetic acid and methanol. Mixing G250 with trichloracetic acid without methanol can create a dye in which gel destaining is no longer required. G250 and R250 have a sensitivity of around 30 ng and 100 ng, respectively. Additionally, GoldBio offers a fantastic alternative to traditional Coomassie stains: the Sapphire Coomassie powder kit, which provides an all-included, ready-to-dissolve powder that can be reused multiple times to stain up to 60 gels and is nearly twice as sensitive as silver staining!

    " ["hide"]=> int(1) ["third_party_id"]=> int(219) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(125) ["root"]=> int(393) ["lft"]=> int(36) ["rgt"]=> int(37) ["level"]=> int(1) ["name"]=> string(22) "Coomassie Stains (old)" ["url_name"]=> string(16) "coomassie-stains" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(825) "

    Coomassie stains are a popular method of protein gel electrophoresis or SDS-PAGE staining. Gold Biotechnology has both G250 (blue) and R250 (red) dyes available for purchase. These dyes bind to proteins to form a protein-dye complex while the polyacrylamide gels can be easily destained with a mixture of acetic acid and methanol. Mixing G250 with trichloracetic acid without methanol can create a dye in which gel destaining is no longer required. G250 and R250 have a sensitivity of around 30 ng and 100 ng, respectively. Additionally, GoldBio offers a fantastic alternative to traditional Coomassie stains: the Sapphire Coomassie powder kit, which provides an all-included, ready-to-dissolve powder that can be reused multiple times to stain up to 60 gels and is nearly twice as sensitive as silver staining!

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  • object(anvilstl\commercio\models\Category)#442 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(30) ["banner_image_id"]=> int(0) ["meta_description"]=> string(150) "DNA and protein tools are vital to molecular research. GoldBio offers ready-to-use ladders, lysis buffers, Coomassie stains and other needed products." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(9) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> string(22) "rgba(221, 187, 121, 1)" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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  • object(anvilstl\commercio\models\Category)#444 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(89) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Biochemical-Reagents.jpg" ["medium"]=> string(90) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Biochemical-Reagents.jpg" ["large"]=> string(89) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Biochemical-Reagents.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(89) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Biochemical-Reagents.jpg" ["medium"]=> string(90) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Biochemical-Reagents.jpg" ["large"]=> string(89) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Biochemical-Reagents.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(201) ["root"]=> int(1) ["lft"]=> int(161) ["rgt"]=> int(180) ["level"]=> int(2) ["name"]=> string(20) "Biochemical Reagents" ["url_name"]=> string(20) "biochemical-reagents" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(545) "GoldBio has a wide variety of analytical and organic reagents to ensure your research is accurate. For testing and classification purposes, we also offer a range of dyes, stains and indicators along with substrates and standards that will help you identify and analyze everything from biochemical interactions to bacterial classifications. In addition, our stock of pharmaceutical intermediates will help you build research grade molecules. All of these products are offered at the highest grade to ensure that you get the most accurate results." ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(25) ["banner_image_id"]=> int(0) ["meta_description"]=> string(122) "GoldBio has a wide variety of biochemical reagents for use to help you create, discover and test successfully in your lab." 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In addition, our stock of pharmaceutical intermediates will help you build research grade molecules. All of these products are offered at the highest grade to ensure that you get the most accurate results." ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(25) ["banner_image_id"]=> int(0) ["meta_description"]=> string(122) "GoldBio has a wide variety of biochemical reagents for use to help you create, discover and test successfully in your lab." 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" ["meta_keywords"]=> string(252) "protein stain, dye, in-gel, gel staining, SDS-PAGE, polyacrylamide, coomassie, western, western blot, sequence, mass spectrophotometry, protein analysis, protein quantification, analysis, protein detection, protein visualization, fixation, fluorescence" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(360) ["root"]=> int(1) ["lft"]=> int(313) ["rgt"]=> int(314) ["level"]=> int(4) ["name"]=> string(20) "Protein Gel Staining" ["url_name"]=> string(20) "protein-gel-staining" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(154) "GoldBio’s in-gel dye-based stains, including our Coomassie stains, allow for protein analysis in SDS-PAGE with a visible blue stain or by fluorescence. 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string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(1) { [0]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" ["slug"]=> string(7) "content" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RichTextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(1000) "

    Detection of proteins immobilized in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is an essential step in Western blots and protein analysis. GoldBio offers a variety of easy-to-use in-gel dye-based stains that diffuse into the gel allowing you to visualize your protein and accurately perform protein quantification. Our Coomassie Brilliant Blue G-250 and R250 dyes produce a robust signal in SDS-PAGE and blue native (BN-PAGE) and are compatible with downstream applications including protein extraction, sequencing and mass spectrometry. We also carry a safer and faster alternative to Coomassie stains, Blazin’ Blue™ Protein Gel Stain, which allows detection of protein through visible blue staining or by near-infrared fluorescence. Alternatively, you can detect your protein with our highly sensitive Blazin’ Bright™ Luminescent Protein Gel Stains, which can stain as little as 1-10 ng of protein in a single step and without the need for fixation.

    " } } } } } } }

    • Coomassie Brilliant Blue G-250

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    Specifications Documentation Related Products
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    Product Description

    Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration. G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.

    Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7). CBBG is soluble in water but methanol and acetic acid are often added for better solubility.

    Product Specifications
    Comassie Brilliant Blue G-250, Ultra Pure
    CBBG, Ultra Pure

    Formula: C47H48N3O7S2Na

    MW: 854.02 g/mol

    Storage/Handling: Store in a dry and well-ventilated place.

    PubChem Chemical ID: 61363

    Product Specifications

    Catalog ID C-460
    CAS # 6104-58-1
    MW 854.02 g/mol
    Storage/Handling Store desiccated at room temperature.

    Technical Documentation

    P Protocol
    O Other
    C Certificate of Analysis
    S Safety Data Sheet
    P
    In Gel Digestion and Extraction of Proteins Protocol

    In Gel Digestion of proteins - including destaining, alkylation, digestion and recovery.
    P
    Bradford Protein Assay

    Bradford protein assay protocol for protein quantitation
    P
    Western Blotting and Immunodetection Protocol

    Western blotting and immunodetection procedure for protein characterization and analysis.

    C
    Sample COA - C-460

    Sample Certificate of Analysis - Coomassie Brilliant Blue G-250
    COA for example only. Actual tests may differ.
    S
    SDS (MSDS) - Coomassie Brilliant Blue G-250

    Safety Data Sheet

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    Coomassie Brilliant Blue R-250
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    C-461-5 5 g $ 30.00
    C-461-25 25 g $ 43.00
    C-461-100 100 g $ 80.00

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