Coomassie stains are a popular method of protein gel electrophoresis or SDS-PAGE staining. Gold Biotechnology has both G250 (blue) and R250 (red) dyes available for purchase. These dyes bind to proteins to form a protein-dye complex while the polyacrylamide gels can be easily destained with a mixture of acetic acid and methanol. Mixing G250 with trichloracetic acid without methanol can create a dye in which gel destaining is no longer required. G250 and R250 have a sensitivity of around 30 ng and 100 ng, respectively. Additionally, GoldBio offers a fantastic alternative to traditional Coomassie stains: the Sapphire Coomassie powder kit, which provides an all-included, ready-to-dissolve powder that can be reused multiple times to stain up to 60 gels and is nearly twice as sensitive as silver staining!
" ["hide"]=> int(1) ["third_party_id"]=> int(219) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(125) ["root"]=> int(393) ["lft"]=> int(36) ["rgt"]=> int(37) ["level"]=> int(1) ["name"]=> string(22) "Coomassie Stains (old)" ["url_name"]=> string(16) "coomassie-stains" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(825) "Coomassie stains are a popular method of protein gel electrophoresis or SDS-PAGE staining. Gold Biotechnology has both G250 (blue) and R250 (red) dyes available for purchase. These dyes bind to proteins to form a protein-dye complex while the polyacrylamide gels can be easily destained with a mixture of acetic acid and methanol. Mixing G250 with trichloracetic acid without methanol can create a dye in which gel destaining is no longer required. G250 and R250 have a sensitivity of around 30 ng and 100 ng, respectively. Additionally, GoldBio offers a fantastic alternative to traditional Coomassie stains: the Sapphire Coomassie powder kit, which provides an all-included, ready-to-dissolve powder that can be reused multiple times to stain up to 60 gels and is nearly twice as sensitive as silver staining!
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quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.
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string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#443 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }Detection of proteins immobilized in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is an essential step in Western blots and protein analysis. GoldBio offers a variety of easy-to-use in-gel dye-based stains that diffuse into the gel allowing you to visualize your protein and accurately perform protein quantification. Our Coomassie Brilliant Blue G-250 and R250 dyes produce a robust signal in SDS-PAGE and blue native (BN-PAGE) and are compatible with downstream applications including protein extraction, sequencing and mass spectrometry. We also carry a safer and faster alternative to Coomassie stains, Blazin’ Blue™ Protein Gel Stain, which allows detection of protein through visible blue staining or by near-infrared fluorescence. Alternatively, you can detect your protein with our highly sensitive Blazin’ Bright™ Luminescent Protein Gel Stains, which can stain as little as 1-10 ng of protein in a single step and without the need for fixation.
Coomassie Brilliant Blue G-250
Product Description
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration. G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.
Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7). CBBG is soluble in water but methanol and acetic acid are often added for better solubility.
Product Specifications
Comassie Brilliant Blue G-250, Ultra Pure
CBBG, Ultra Pure
Formula: C47H48N3O7S2Na
MW: 854.02 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 61363
Product Specifications
Catalog ID | C-460 |
---|---|
CAS # | 6104-58-1 |
MW | 854.02 g/mol |
Storage/Handling | Store desiccated at room temperature. |