Coomassie Brilliant Blue G-250
Product Description
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration. G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.
Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7). CBBG is soluble in water but methanol and acetic acid are often added for better solubility.
Product Specifications
Comassie Brilliant Blue G-250, Ultra Pure
CBBG, Ultra Pure
Formula: C47H48N3O7S2Na
MW: 854.02 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 61363
Product Specifications
| Catalog ID | C-460 |
|---|---|
| CAS # | 6104-58-1 |
| MW | 854.02 g/mol |
| Storage/Handling | Store desiccated at room temperature. |
Technical Documentation
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