MOPS Free Acid, Ultra Pure

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Product Description

MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.

MOPS free acid can be mixed with MOPS sodium salt to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.

Product Specifications
MOPS, Ultra Pure
3-(N-morpholino)propanesulfonic acid

Formula: C7H15NO4S

MW: 209.26 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 70807

Product Specifications

Catalog ID M-790
CAS # 1132-61-2
MW 209.26 g/mol
Storage/Handling Store desiccated at room temperature.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet
P
RNA Gel and Northern Blotting Protocol

Protocol for RNA Gel and Northern Blotting - adapted from Wolf Lab, Sanford-Burnham Medical Research Institute.
P
MOPS Running Buffer Preparation Protocol

10X MOPS Running Buffer Preparation protocol, pH 7.0 (200mM MOPS)
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MOPS Buffer Stock Solution

MOPS Stock Solution - 1M
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Printable Buffer Chart - Arranged by Family

This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
O
Printable Buffer pH Chart - Arranged In Order of pH

Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
O
User Guide for GoldBio Buffers

A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
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Biological Buffer Selection Guide

The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.

C
Sample COA - M-790

Sample Certificate of Analysis - MOPS, Ultra Pure
COA for example only. Actual tests may differ.
S
SDS (MSDS) - MOPS

Safety Data Sheet

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