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Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

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Tris HCl

Name: Tris HCl
SKU: T-095
Availability: InStock

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Product Description

Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

A Tris buffer solution can be made by mixing Tris with Tris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases. If neutralizing Tris-HCl with a strong base such as NaOH to make a buffer solution, ionic strength should be taken into consideration because of the formation of salt.

Product Specifications
Tris Hydrochloride
Tris HCl;
2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride

Formula: C₄H₁₁NO₃ · HCl

MW: 157.60 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 93573

Product Specifications

Catalog ID	T-095
CAS #	1185-53-1
MW	157.60 g/mol
Storage/Handling	Store desiccated at room temperature.

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

DNase Inactivation in RNase A Solution Protocol

Protocol for DNase inactivation in RNase A solution.

IPTG Induction and Extraction of Proteins Protocol

IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis

Tris HCl Buffer Stock Solution

Tris HCl Buffer Stock Solution - 1M

Electrotransformation of Agrobacterium tumefaciens

Use of electroporation to modify Agrobacterium tumefaciens.

Radio Immunoprecipitation Assay (RIPA) Cell Lysate Preparation

Use of RIPA Buffer to prepare cell lysates for protein assays, protein purification and other downstream applications.

User Guide for GoldBio Buffers

A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.

Biological Buffer Selection Guide

The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.

Sample COA - T-095

Sample Certificate of Analysis - Tris HCl
COA for example only. Actual tests may differ.

SDS (MSDS) - Tris HCl

Safety Data Sheet