Cefotaxime (Sodium) 250 mg/mL Solution
Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria. Cefotaxime is often used in Agrobacterium tumefaciens-mediated transformations to regulate bacterial growth. It has been shown to exhibit a synergistic effect with Vancomycin in certain conditions.
Cephalosporins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a six-membered dihydrothiazine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Cephalosporins are less susceptible to β-lactamases than the penicillin β-lactam antibiotics.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
GoldBio’s antibiotic solutions are provided ready to use.
Cefotaxime Sodium in H 2O
250 mg/mL Premade Solution, 0.2 µm Sterile Filtered
MOLECULAR BIOLOGY GRADE
Formula: NaC 16H16N5O7S2
MW: 477.45 g/mol
Storage/Handling: Store at -20°C.
PubChem Chemical ID: 10695961
|Grade||MOLECULAR BIOLOGY GRADE|
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Examine the Effect of Carbenicilln, Cefotaxime and Vancomycin on Plant Morphogenesis by Teixeira da Silva, JA and S. Fukai (2001)
Sample Certificate of Analysis - Cefotaxime
COA for example only. Actual tests may differ.