5-Fluoroorotic acid monohydrate (5-FOA)

Request Lot Specific COA

View Current Lot COA

Citation Search Tool

Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications:
5-Fluoroorotic acid monohydrate (5-FOA)
5-Fluorouracil-4-carboxylic acid

MOLECULAR BIOLOGY GRADE

Formula: C5H3FN2O4 • H2O

MW: 192.10 g/mol

White to slightly off-white crystalline powder

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 16212749

5-Fluoroorotic acid monohydrate (5-FOA)

View Sizes & Pricing

Catalog Number:
F-230-2.5
CAS Number:
220141-70-8
$111.00

For research use only. Not for food, drug, household, or cosmetic use.
Availability:
In stock
Shipping:
$14.99 Ground shipping (In continental US only.)

    Description

    5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

    Product Specifications:
    5-Fluoroorotic acid monohydrate (5-FOA)
    5-Fluorouracil-4-carboxylic acid

    MOLECULAR BIOLOGY GRADE

    Formula: C5H3FN2O4 • H2O

    MW: 192.10 g/mol

    White to slightly off-white crystalline powder

    Storage/Handling: Store at -20°C. Protect from light.

    PubChem Chemical ID: 16212749

    Product Specifications

    Catalog ID: F-230
    CAS #: 220141-70-8
    Formula: C5H3FN2O4•H2O
    MW: 192.10 g/mol
    Grade: MOLECULAR BIOLOGY GRADE
    Storage/handling: Store at -20°C. Protect from light.
    PubChem Chemical ID: 16212749
    IUPAC Name: 5-fluoro-2,4-dioxo-1H-pyrimidine-6-carboxylic acid;hydrate
    InChI: InChI=1S/C5H3FN2O4.H2O/c6-1-2(4(10)11)7-5(12)8-3(1)9;/h(H,10,11)(H2,7,8,9,12);1H2
    InChIKey: LODRRYMGPWQCTR-UHFFFAOYSA-N
    SMILES: C1(=C(NC(=O)NC1=O)C(=O)O)F.O

    CITATIONS

    Featured Articles

      Login

      Forgot your password?

      Don't have an account yet?
      Create account