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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget. Peruse below for our most popular biological buffers! Click here for the comprehensive selection of GoldBio buffers.

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  • Product Description

    BES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is a bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 6.4 – 7.8. It is soluble in water at 0°C up to 3.2M. Like most neutral, amine-based buffers, BES forms a complex with DNA. It interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in BES shields the negative charge of DNA to a lesser extent than less substituted buffers. BES can be used in a buffered saline solution in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. BES also forms a complex with certain metals, including copper and cobalt. Though BES binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

    Product Specifications
    BES;
    N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid;
    N,N-Bis(2-hydroxyethyl)taurine

    ULTRA PURE GRADE

    Formula: C6H15NO5S

    MW: 213.25 g/mol

    PubChem Chemical ID: 73243

    Storage/Handling: Store at room temperature.

    Product Specifications

    Catalog ID B-780
    CAS # 10191-18-1
    MW 213.25 g/mol
    Grade ULTRA PURE GRADE
    Storage/Handling Store at room temperature.

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