Description
The ReadyLadder™ 1 kb DNA Ladder from GoldBio offers a convenient, ready-to-use solution for accurate DNA sizing in agarose gel electrophoresis. Featuring 13 defined bands spanning 250 bp to 10 kb, it provides the resolution needed for routine experiments.
For easy visualization, the 1 kb and 3 kb bands are presented with greater intensity, ensuring fast orientation on the gel. With each load containing 0.5 μg of DNA, users can not only determine fragment size but also estimate DNA quantity by comparing band intensity. Premixed with Xylene cyanol FF and Bromophenol blue tracking dyes, this ladder simplifies workflows by allowing direct gel loading and real-time tracking of DNA migration. Reliable, consistent, and stable during shipping and storage, it’s an essential tool for accurate DNA analysis.
Common Research Applications
(Click each for more information)
Estimation of DNA Fragment Sizes During Gel Electrophoresis
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Purpose: Serves as a molecular weight marker to estimate the size of DNA fragments.
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How It Works: Contains DNA fragments from 250 bp to 10 kb, spaced at 1 kb intervals. When run alongside experimental DNA, the ladder provides reference points for fragment size estimation.
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Applications: Agarose gel-based size validation for PCR products, digested DNA, or cloned constructs.
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
Verification of PCR Amplification Products
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Purpose: Confirms successful PCR amplification and fragment integrity.
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How It Works: PCR products are compared against the 1 kb ladder to verify expected size and detect nonspecific amplification.
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Applications: Gene expression studies, genotyping, microbial identification, and mutational analysis.
Dieffenbach, C. W., & Dveksler, G. S. (1995). PCR Primer: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
Quality Control of Restriction Enzyme Digests
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Purpose: Verifies successful DNA digestion using restriction enzymes.
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How It Works: Digested DNA is run alongside the ladder to confirm expected fragment sizes and digestion efficiency.
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Applications: Cloning workflows, construct validation, and vector mapping.
Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press.
Screening and Confirmation of Insert Presence During Cloning
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Purpose: Confirms whether a DNA insert has been successfully cloned into a vector.
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How It Works: Colony PCR or restriction digest products are analyzed alongside the ladder to verify insert presence and approximate size.
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Applications: Molecular cloning, synthetic biology, and CRISPR construct validation.
Mumberg, D., Müller, R., & Funk, M. (1995). Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene, 156(1), 119–122.
Gel-Based Genotyping and Molecular Marker Analysis
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Purpose: Assists in detecting genetic polymorphisms by comparing PCR product sizes.
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How It Works: Marker PCR products are sized against the ladder to identify allele-specific banding patterns.
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Applications: Plant and animal breeding, QTL mapping, population genetics, and molecular diagnostics.
Varshney, R. K., Graner, A., & Sorrells, M. E. (2005). Genic microsatellite markers in plants: Features and applications. Trends in Biotechnology, 23(1), 48–55.
Benefits
- Sharp, clear bands for reliable sizing
- Quick reference with enhanced bands (3 kb, 1 kb)
- Ready-to-use—premixed with loading dye for direct loading
- Stable for shipping at room temperature
Specifications
Range: 250bp - 10kb
Number of bands: 13
Loading dye contains: Xylene cyanol FF and Bromophenol Blue as tracking dyes.
Recommended Load:
5 μl/well (0.5 μg)
Storage/Handling:
Stable for shipping at ambient temperatures
Store at 25°C for 6 months.
Store at -20°C for 24 months.
Comparison between ReadyLadder™1 kb and ReadyLadder™1 kb PLUS™ ladders
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