Description GoldBio’s ReadyLadder™ 100 bp DNA Ladder is a convenient, ready-to-use molecular weight marker designed for accurate sizing of small DNA fragments. Composed of 11 defined bands ranging from 100 bp to 1,500 bp, this ladder is ideal for applications requiring high resolution of low–molecular weight DNA.
The 1,500 bp and 500 bp bands are intensified, giving you clear visual markers that make gel interpretation straightforward. With 0.5 μg of DNA per load, the ladder not only supports accurate fragment size determination but also provides a convenient way to estimate sample quantities by comparing band intensity. Because it is premixed with Orange G and Xylene cyanol FF tracking dyes, you can load it directly onto the gel and follow migration in real time. This ladder is designed for sharp, reproducible banding and long-term stability.
Loading dye contains orange G & xylene cyanol FF as tracking dyes.
Common Research Applications (Click each for more information)
Size Estimation of DNA Fragments in PCR and Cloning Workflows
Purpose : Used to determine the length of double-stranded DNA fragments during agarose gel electrophoresis.
How It Works : The 100 bp ladder provides 11 distinct bands ranging from 100 to 1,500 bp. Sample bands are run alongside the ladder, and fragment sizes are estimated by comparing migration distances.
Applications : PCR validation, genotyping, amplicon profiling, and insert screening in cloning workflows.
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press.
Integrity Verification of PCR Products (Amplicon Analysis)
Purpose : Confirms that PCR amplification generated the correct-sized product and reveals the presence of primer dimers or nonspecific bands.
How It Works : PCR products are electrophoresed beside the 100 bp ladder to determine whether the band corresponds to the expected size.
Applications : Diagnostic assays, gene expression studies, microbial identification, and sequencing preparation.
Bustin, S. A., et al. (2009). The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry , 55(4), 611–622.
Validation of Restriction Enzyme Digests
Purpose : Confirms expected cleavage patterns in plasmids or genomic DNA after restriction digestion.
How It Works : DNA fragments generated by enzyme digestion are run alongside the 100 bp ladder, enabling verification of expected fragment sizes and proper enzyme function.
Applications : Plasmid mapping, insert verification, and confirming synthetic construct assembly.
Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press.
Screening and Confirmation of Insert Presence During Cloning
Purpose : Detects presence or absence of inserts in recombinant plasmids using colony PCR or restriction digest analysis.
How It Works : DNA fragments from clones are compared against the ladder to confirm the presence and size of the insert.
Applications : Vector construction, gene expression studies, and mutagenesis workflows.
Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press.
Electrophoresis System Calibration and Gel Optimization
Purpose : Optimizes gel resolution, agarose concentration, and buffer conditions for DNA separation.
How It Works : A known, evenly spaced banding pattern enables users to assess separation quality, confirm voltage parameters, and troubleshoot gel system performance.
Applications : Setting up new gel rigs, switching buffer systems, or troubleshooting smeared or unclear bands.
Benefits
Sharp, consistent bands for precise sizing
Quick reference with enhanced bands (1,500 bp, 500 bp)
Ready-to-use—premixed with loading dye for direct loading
Stable for shipping at room temperature
Specifications
Loading dye contains orange G & xylene cyanol FF as tracking dyes.
Range : 100bp - 1500bp
Number of bands : 11
Loading dye contains : orange G & xylene cyanol FF as tracking dyes.
Recommended Load
5 μl/well (0.5 μg)
Storage/Handling:
Stable for shipping at ambient temperatures
Comparison between ReadyLadder™ 100 bp and ReadyLadder™ 100 bp PLUS ladders
Guide to Choosing Your DNA Ladder
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