Description
GoldBio’s 4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate widely used for the detection, characterization, and kinetic analysis of α-glucosidase activity in biochemical assays. Upon enzymatic cleavage by α-glucosidase enzymes, the substrate releases the fluorescent compound 4-methylumbelliferone (4-MU), enabling sensitive fluorescence-based quantification of enzymatic activity. This makes the reagent particularly valuable for glycosidase research, lysosomal enzyme studies, inhibitor screening, and metabolic disorder investigations.
The product is commonly used in assays involving acid α-glucosidase, including research associated with Pompe disease and related glycogen storage disorders. Because fluorescence-based detection provides high sensitivity and sensitive fluorescence-based detection., 4-Methylumbelliferyl-α-D-glucopyranoside is frequently incorporated into high-throughput screening assays and enzyme characterization studies.
GoldBio supplies this fluorogenic substrate for research applications requiring reproducibility, sensitive signal detection, and compatibility with established α-glucosidase assay systems.
Common Research Applications
(Click each for more information)
Fluorogenic Detection of α-Glucosidase Activity
-
Purpose: To sensitively measure α-glucosidase enzyme activity in biochemical assays.
-
How It Works: Enzymatic cleavage of 4-Methylumbelliferyl-α-D-glucopyranoside releases fluorescent 4-methylumbelliferone, which can be quantified fluorometrically.
-
Applications: Enzyme kinetics, glycosidase characterization, and comparative activity studies.
Bravo-Torres, J. C., Villagómez-Castro, J. C., Calvo-Méndez, C., Flores-Carreón, A., & López-Romero, E. (2004). Purification and biochemical characterisation of a membrane-bound alpha-glucosidase from the parasite Entamoeba histolytica. International Journal for Parasitology, 34(4), 455–462. PMID: 15013735.
High-Throughput Screening of α-Glucosidase Inhibitors
-
Purpose: To identify and characterize compounds that inhibit α-glucosidase activity.
-
How It Works: Reduced fluorescent signal indicates inhibition of substrate hydrolysis by test compounds.
-
Applications: Drug discovery research, natural product screening, and enzyme inhibitor analysis.
Matsui, T., Tanaka, T., Tamura, S., Toshima, A., Miyata, Y., Takeuchi, K., & Matsumoto, K. (2009). α-Glucosidase inhibition assay in an enzyme-immobilized amino-microplate. Analytical Sciences, 25(4), 559–562. PMID: 19359799.
Microplate-Based Glycosidase Assays
-
Purpose: To enable rapid fluorescent detection in multi-sample assay formats.
-
How It Works: The fluorogenic substrate produces measurable fluorescence compatible with microplate readers.
-
Applications: High-throughput enzymology, screening assays, and automated fluorescence workflows.
Motabar, O., Shi, Z.-D., Goldin, E., Liu, K., Southall, N., Sidransky, E., Austin, C. P., Griffiths, G. L., & Zheng, W. (2009). A new resorufin-based alpha-glucosidase assay for high-throughput screening. Analytical Biochemistry, 390(1), 79–84. PMID: 19371716.
Key Benefits:
- Sensitive fluorescence-based detection of α-glucosidase activity.
- Useful for kinetic analysis, enzyme characterization, and inhibitor screening studies.
- Compatible with established lysosomal enzyme and glycosidase assay methods.
- Supports high-throughput screening applications through rapid fluorescent readouts.
- Fluorogenic substrate soluble in DMSO and other organic solvents, with limited direct aqueous solubility; typically dissolved in DMSO before dilution into assay buffer.
Powered by Bioz