Biotin‐PEG4‐NHS Ester

Request Lot Specific COA

Citation Search Tool

Description

Biotin‐PEG4‐NHS Ester is a pegylated, highly hydrophilic biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing biomolecules.

Biotin‐PEG4‐NHS Ester reacts specifically and efficiently with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Formula: C25H40N4O10S

MW: 588.67 g/mol

Net Mass Addition: 473.22 g/mol

Spacer Arm: 29Å

Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in water at 10 mg/ml.

Highlights:
- A highly hydrophilic biotinylation reagent amine reactive biotinylation reagent.
- Biotin‐PEG4‐NHS Ester increases solubility and eliminates aggregation of labeled molecules.
- Extended PEG4 spacer arm reduces steric hindrance for optimal streptavidin binding.
- High purity, solid biotin labeling reagents can be used to create high-quality biotin-labeled derivatives
biotinylation reagents

Biotin‐PEG4‐NHS Ester

View Sizes & Pricing

Catalog Number:
B-720-25
CAS Number:
$128.00

Availability:
In stock
Shipping:
Shipping calculated at checkout

    Description

    Biotin‐PEG4‐NHS Ester is a pegylated, highly hydrophilic biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing biomolecules.

    Biotin‐PEG4‐NHS Ester reacts specifically and efficiently with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

    Formula: C25H40N4O10S

    MW: 588.67 g/mol

    Net Mass Addition: 473.22 g/mol

    Spacer Arm: 29Å

    Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
    Soluble in water at 10 mg/ml.

    Highlights:
    - A highly hydrophilic biotinylation reagent amine reactive biotinylation reagent.
    - Biotin‐PEG4‐NHS Ester increases solubility and eliminates aggregation of labeled molecules.
    - Extended PEG4 spacer arm reduces steric hindrance for optimal streptavidin binding.
    - High purity, solid biotin labeling reagents can be used to create high-quality biotin-labeled derivatives
    biotinylation reagents

    Product Specifications

    Catalog ID: B-720
    Formula: C25H40N4O10S
    MW: 588.67 g/mol
    Storage/handling: Store at -20°C.

    Featured Articles

    • How to Biotinylate Proteins for Experiments and Purification

      How to Biotinylate Proteins for Experiments and Purification

      The protein streptavidin clamps onto the small molecule biotin in one of the tightest natural interactions ever discovered. Over the years, the strength of this...

      Read more

    • labeling proteins - Thumbnail Image

      Everything About Labeling Proteins with NHS Esters

      Handles are common in everyday life. We use them to help us open doors, cabinets, or to hold coffee mugs, cookware, and tools. They are...

      Read more

    Login

    Forgot your password?

    Don't have an account yet?
    Create account