BLUEstain™ 2 Protein ladder, 5-245 kDa

Request Lot Specific COA

Citation Search Tool

Description

  • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 μl per well for general Western transferring.
  • Apply more for thicker (>1.5 mm) or larger gel.
  • Ready-to-use! No dilutions necessary

The BLUEstainTM 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

The BLUEstainTM 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

Storage/Handling: Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.

Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).

BLUEstain™ 2 Protein ladder, 5-245 kDa

View Sizes & Pricing

Catalog Number:
P008-500
CAS Number:
$120.00

Availability:
In Stock
Shipping:
Shipping calculated at checkout

    Description

    • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
    • 1.5~2.5 μl per well for general Western transferring.
    • Apply more for thicker (>1.5 mm) or larger gel.
    • Ready-to-use! No dilutions necessary

    The BLUEstainTM 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

    The BLUEstainTM 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

    Storage/Handling: Store at 4°C for 3 months. Store at -20°C for 24 months.
    This product is stable for shipping at ambient temperatures.

    Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).

    Product Specifications

    Catalog ID: P008
    Storage/handling: Store at 4°C for 3 months. Store at -20°C for 24 months.

    CITATIONS

    Featured Articles

    • Protein Electrophoresis Using SDS-PAGE: A Detailed Overview

      Protein Electrophoresis Using SDS-PAGE: A Detailed Overview

      Each protein has a specific molecular weight. This property is used by scientists to separate them based on their sizes. SDS-PAGE is a technique to...

      Read more

    • Protein Ladders: Prestained Versus Unstained

      Protein Ladders: Prestained Versus Unstained

      Two categories of commercially available protein ladders fall under being unstained protein ladders and prestained protein ladders. And, if you're wanting to know what the...

      Read more

    Login

    Forgot your password?

    Don't have an account yet?
    Create account