Description
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (>1.5 mm) or larger gel.
- Ready-to-use! No dilutions necessary
GoldBio’s BLUEstain™ Protein Ladder (11–245 kDa) is a tri-color, pre-stained molecular weight marker designed for high-resolution SDS-PAGE and Western blot applications. It contains 12 highly visible protein bands, spanning ~11 to 245 kDa, allowing for accurate molecular weight estimation, transfer validation, and gel run monitoring. With blue, green, and orange color-coded bands for easy reference, BLUEstain™ ensures real-time visibility during electrophoresis and membrane transfer. This ready-to-use ladder streamlines workflows, eliminates the need for staining, and supports consistent documentation across experiments. Ideal for research labs seeking fast, reliable protein analysis, BLUEstain™ sets the standard in gel-based proteomics.
Contents:
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2mM Dithiothreitol, 3.6M Urea, and 15% (v/v) Glycerol).
Storage/Handling:
Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.
Common Applications:
(Click each for more information)
Molecular Weight Estimation via SDS-PAGE
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Purpose: To determine the molecular weight of proteins separated by electrophoresis.
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How It Works: The BLUEstain™ Protein Ladder features 12 pre-stained bands spanning approximately 11 to 245 kDa. These visible reference bands migrate alongside experimental samples during SDS-PAGE, allowing estimation of target protein molecular weight based on relative migration.
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Applications: Protein purification validation, antibody characterization, and recombinant protein verification.
Ladner, C. L., et al. (2004). Visible fluorescent detection of proteins in polyacrylamide gels without staining. Analytical Biochemistry, 326(1), 13–20.
Verification of Protein Transfer in Western Blotting
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Purpose: To confirm successful and uniform protein transfer from gel to membrane.
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How It Works: The pre-stained BLUEstain™ bands remain visible after electroblotting, serving as transfer markers across the molecular weight range.
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Applications: Western blot quality control, transfer efficiency assessment, and membrane orientation verification.
Mahmood, T., & Yang, P. C. (2012). Western blot: Technique, theory, and troubleshooting. North American Journal of Medical Sciences, 4(9), 429–434.
Real-Time Monitoring of Gel Electrophoresis
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Purpose: To monitor protein migration and electrophoresis progress during SDS-PAGE runs.
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How It Works: Visible pre-stained bands allow users to track migration in real time, helping optimize run duration and prevent proteins from running off the gel.
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Applications: Electrophoresis timing control, troubleshooting, and live visualization during gel runs.
Ramos-Vara, J. A. (2005). Technical aspects of immunohistochemistry. Veterinary Pathology, 42(4), 405–426.
Training and Protocol Optimization
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Purpose: To provide visual feedback during training and assay development.
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How It Works: BLUEstain™’s clearly visible colored bands help validate gel composition, electrophoresis settings, and buffer performance during method optimization.
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Applications: Laboratory training, SOP refinement, and troubleshooting of new electrophoresis systems.
Matsudaira, P. (1987). Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry, 262(21), 10035–10038.
Cross-Gel Band Alignment and Transfer Consistency Checks
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Purpose: To ensure reproducibility across multiple SDS-PAGE or Western blot experiments.
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How It Works: Using the same ladder across gels provides a standardized molecular weight reference for comparing migration and transfer consistency between experiments.
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Applications: Comparative protein analysis, reproducibility studies, and QC in high-throughput workflows.
Neuhoff, V., et al. (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity. Electrophoresis, 9(6), 255–262.
Benefits:
- High Band Resolution Across Full Size Range: Covers both small peptides and large proteins with 12 clearly defined bands , enabling broad application across many research targets.
- Immediate Visualization: Bands are visible during runs and transfer—no staining, destaining, or development needed.
- Time-Saving Format: Reduces time in gel prep, visualization, and validation compared to post-stain workflows.
- Reliable Transfer Confirmation: Verifies blot transfer success without relying solely on antibody-based detection.
- Supports Standardization: Improves data reproducibility across labs and experimental runs.
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