Description
GoldBio's Reagent Grade, Fatty Acid Free BSA is ideally suited for fatty acid sensitive cell culture systems, such as CHO, Vero and MDCK cells, as well as for the most sensitive diagnostic and research applications.
Our BSA is also suitable for Western blots, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments.
This Bovine Serum Albumin (BSA), Fraction V, Fatty Acid-Free is a high-purity, protease-free, IgG-free, low-endotoxin protein carrier engineered for lipid-sensitive research.
With ≥98% purity and ≤0.01 U/mg non-esterified fatty acids, it delivers unmatched performance in lipid metabolism studies, serum-free culture systems, ligand-binding assays, and hydrophobic molecule delivery.
FA-free BSA ensures precise experimental control by eliminating background lipid interference, making it indispensable for fatty acid delivery models, and lipid-sensitive immunoassays.
Manufactured in the USA from USDA-inspected herds and TSE-free certified, GoldBio’s FA-free BSA provides the reliability, consistency, and quality demanded by modern molecular biology and biotechnology research.
The BSA protein is a single polypeptide chain consisting of ~583 amino acid residues and no carbohydrates and is approximately 66 kDa in size. Fraction V refers to the fifth fraction of the original Cohn purification of plasma proteins using cold ethanol precipitation and is still commonly used as a name for bovine serum albumin regardless of purification method.
GoldBio BSA is protease free and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.
REAGENT GRADE / FATTY ACID FREE / PROTEASE FREE / IgG FREE
Common Research Applications:
(Click each for more information)
Defined Fatty-Acid Delivery for Lipid Metabolism and Lipotoxicity Models
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Purpose: Provide precise and reproducible delivery of long-chain fatty acids to cultured cells without background lipid contamination.
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How It Works: Fatty acids are complexed to fatty acid-free BSA to enhance aqueous solubility and control unbound fatty acid concentration, which governs cellular uptake and signaling.
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Applications: Lipotoxicity studies in β-cells and neurons, fatty acid oxidation assays, lipid-regulated signaling, and post-translational modifications such as acylation.
Listenberger, L. L., Ory, D. S., & Schaffer, J. E. (2001). Palmitate-induced apoptosis can occur through a ceramide-independent pathway. Journal of Biological Chemistry, 276(18), 14890–14895.
Serum-Free or Defined Media Supplement in Lipid-Sensitive Cell Cultures
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Purpose: Support cell growth and viability in serum-free or chemically defined media without introducing undefined lipid species.
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How It Works: Fatty acid-free BSA maintains albumin’s carrier, antioxidant, and osmoprotective roles while avoiding lipids that could alter experimental outcomes.
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Applications: Maintenance of lipid-sensitive primary cells, stem cells, and metabolic model systems under defined conditions.
Francis, G. L. (2010). Albumin and mammalian cell culture: Implications for biotechnology applications. Cytotechnology, 62(1), 1–16.
Ligand-Binding and Protein Interaction Studies Without Lipid Interference
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Purpose: Measure accurate binding kinetics for small molecules, peptides, or proteins without competition from endogenous fatty acids.
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How It Works: Fatty acid-free BSA eliminates background fatty acids that could occupy binding sites, ensuring experimental ligands interact as intended.
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Applications: Hormone–protein binding assays, drug–albumin interaction studies, receptor–ligand characterization, and equilibrium dialysis experiments.
Richieri, G. V., & Kleinfeld, A. M. (1995). Unbound free fatty acid levels in human serum. Journal of Biological Chemistry, 270(29), 17223–17229.
Carrier Protein for Hormone, Vitamin, or Drug Delivery Studies
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Purpose: Solubilize, stabilize, and deliver hydrophobic bioactive molecules without interference from pre-bound fatty acids.
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How It Works: Albumin’s hydrophobic binding pockets protect cargo from aggregation and degradation, while fatty acid-free status prevents displacement by endogenous ligands.
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Applications: Delivery of steroid hormones, lipid-soluble vitamins (A, D, E, K), and hydrophobic drugs in vitro.
Curry, S., Mandelkow, H., Brick, P., & Franks, N. (1998). Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites. Nature Structural Biology, 5(9), 827–835.
Blocking Reagent in Immunoassays for Lipid-Sensitive Targets
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Purpose: Reduce non-specific binding in immunoassays where lipid interactions could interfere with antigen or antibody function.
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How It Works: Fatty acid-free BSA coats assay surfaces to prevent nonspecific protein adsorption while avoiding lipid–protein interactions that can alter immune recognition.
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Applications: ELISA, Western blotting, and microarrays involving lipid-binding proteins, membrane receptors, or lipid-modified antigens.
Ströhle, G., & Li, H. (2023). Comparison of blocking reagents for antibody microarray-based immunoassays on glass and paper membrane substrates. Analytical and Bioanalytical Chemistry, 415(8), 1967–1977.
Applications
- Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent
- Protein Standard, Diluent
- Protein, Conjugate or Enzyme Stabilizer
- Hybridization, nucleic acid-based assays Fatty acid sensitive cell culture systems
Benefits
- FA-free specificity: Eliminates lipid background interference in binding, delivery, and metabolic assays.
- High reproducibility: Tight specifications ensure consistent batch-to-batch performance.
- Low endotoxin & contaminant profile: Safe for sensitive mammalian cell cultures.
- Versatile use: Functions as a carrier, stabilizer, blocking agent, and protein standard.
- Regulatory clarity: Manufactured in the USA from USDA-inspected herds; TSE-free source material.
- Long shelf life: Lyophilized powder format with excellent stability under recommended storage.
Specifications
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Protein (on Dried Basis)
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≥98.0%
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Purity (albumin)
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≥98.0%
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Moisture
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≤5.0%
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pH (10% in Water)
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6.5 to 7.5
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Ash (Residue on Ignition)
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<2.0%
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Protease (Enzymatic Assay)
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None Detected
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Non-Esterified Fatty Acids (NEFA)
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<0.01 Units/mg
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Endotoxin
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≤1 EU/mg
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Heavy Metals (Pb)
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≤10 ppm
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IgG
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None Detected
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Storage/Handling
Store at 4°C.
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