Description
GoldBio's Caprylate Stabilized BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments.
Caprylate stabilized BSA has exceptional purity and low interference, utilizing heat-shock fractionation designed to inactivate proteases and other potentially interfering enzymes.
BSA protein is a single polypeptide chain consisting of ~583 amino acid residues and no carbohydrates and is approximately 66 kDa in size. Fraction V refers to the fifth fraction of the original Cohn purification of plasma proteins using cold ethanol precipitation and is still commonly used as a name for bovine serum albumin regardless of purification method.
GoldBio BSA is protease and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.
Common Research Applications for Bovine Serum Albumin (BSA), Fraction V, Protease Free
(Click each for more information)
Blocking Agent in Immunoassays (ELISA, Western Blot, Immunohistochemistry)
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Purpose: Prevents nonspecific binding of antibodies to assay surfaces.
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How It Works: BSA saturates unoccupied binding sites on plates, membranes, or tissues, minimizing background signal by blocking nonspecific protein interactions.
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Applications: ELISA, Western blotting, immunocytochemistry, and immunofluorescence.
Lequin, R. M. (2005). Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clinical Chemistry, 51(12), 2415–2418.
Stabilizer and Carrier Protein for Enzymes and Antibodies
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Purpose: Maintains the integrity and biological activity of proteins in solution by preventing aggregation and surface adsorption.
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How It Works: BSA binds nonspecifically to hydrophobic surfaces and reactive groups, forming a protective colloid and stabilizing labile biomolecules.
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Applications: Enzyme assays, antibody storage, lyophilization buffers, and diagnostic reagents.
Arakawa, T., & Timasheff, S. N. (1985). Stabilization of protein structure by sugars. Biochemistry, 24(25), 6756–6762.
Protein Standard in Quantification Assays (BCA, Bradford, Lowry)
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Purpose: Serves as a reliable and consistent standard for protein concentration determination.
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How It Works: BSA’s consistent composition enables reproducible dye binding in colorimetric assays such as BCA, Bradford, and Lowry.
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Applications: Generation of standard curves and calibration of spectrophotometric protein assays.
Kruger, N. J. (2009). The Bradford method for protein quantitation. In The Protein Protocols Handbook (pp. 17–24). Humana Press.
Cell Culture Supplement and Serum Replacement
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Purpose: Enhances cell viability, growth, and function in low-serum or serum-free media.
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How It Works: BSA binds toxins, stabilizes vitamins and hormones, and supplies nutrients, helping mimic serum-like conditions in defined media.
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Applications: Mammalian cell culture, stem cell maintenance, and hybridoma development.
Jayme, D. W., & Smith, S. R. (2000). Media formulation options and manufacturing process controls to safeguard cell culture performance. Bioprocessing Journal, 1(1), 49–56.
Blocking Agent in Flow Cytometry Buffers
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Purpose: Prevents nonspecific antibody binding to Fc receptors or other cellular components during immunostaining.
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How It Works: BSA is included in staining and wash buffers to block background interactions, improving staining specificity and signal clarity.
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Applications: FACS buffer formulations for immune profiling, intracellular staining, and cell sorting.
Maecker, H. T., & Trotter, J. (2006). Flow cytometry controls, instrument setup, and the determination of positivity. Cytometry Part A, 69A(9), 1037–1042.
Applications
- Western Blotting
- Protein Standard
- Hybridizations
- Immunoassays
- Molecular Biology standard
- Cell Culture media
Benefits
- Reduces background noise in immunoassays and flow cytometry via effective surface blocking.
- Caprylate-stabilized formulation offers enhanced purity and long-term protein stability, reducing oxidative degradation and metal-catalyzed aggregation.
- Protease-free formulation protects labile proteins during stabilization, storage, and assay development.
- Highly pure and endotoxin-tested, suitable for sensitive applications including serum-free cell culture.
- Multipurpose use across quantitation assays, buffer systems, and diagnostic workflows.
- Reproducibility and reliability in experimental workflows due to consistent purity and formulation.
Specifications
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Protein (on Dried Basis)
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≥98.0%
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Purity (albumin)
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≥98.0%
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Moisture
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≤5.0%
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pH (10% in Water)
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6.5 to 7.5
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Ash (Residue on Ignition)
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<2.0%
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Protease (Enzymatic Assay)
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≤0.001 Units/mg
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Endotoxin
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≤1 EU/mg
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Heavy Metals (Pb)
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≤10 ppm
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IgG
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None Detected
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Storage/Handling
Store at 4°C.
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