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Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Product Specifications
| Catalog ID: | R-203 |
|---|---|
| Storage/handling: | Store at 4°C. Do NOT freeze. |
Technical Documentation
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Protocol
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Other
C
Certificate of Analysis
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Safety Data Sheet
CITATIONS
Featured Articles
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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Conjugating Ligands to Agarose Beads for Affinity Purification
Affinity purification is a frequently used technique for purifying proteins. During affinity purification, affinity tags or intrinsic properties of the proteins are used to bind...
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How Agarose Beads are Made
Agarose beads are a foundational tool used for various protein purification techniques. Most frequently, agarose beads are purchased through a vendor such as GoldBio. However,...
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How to Choose Agarose Resins for Protein Purification
Purifying a target protein is an important step before investigating its structure and function. To purify a target protein from a mixture of substances, column...
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How Column Chromatography Works to Separate Proteins
Without a doubt, protein purification has been an important process in protein research, particularly for investigating the function and structure of a protein. To help...
