Cobalt HTC Agarose Beads

Request Lot Specific COA

Citation Search Tool

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

The maximum flow rate at 15 cm bed height is >1000 cm/h and the maximum pressure is >300 kPa. The binding capacity with an approximately 30 kDA protein is 5 mg/mL.

These products are excellent for large scale His-tagged protein purification:

  • Easy scale up and robust function
  • High chemical and physical stability
  • Good resolution in minimum time

HTC beads are high throughput beads designed to meet the demand for industrial process separation, suitable for use with FPLC or HPLC, and can withstand pressures up to 300 kPa. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Storage/Handling: Store at 4°C. Do not freeze.

Cobalt HTC Agarose Beads

View Sizes & Pricing

Catalog Number:
R-203-25
CAS Number:
$325.00

For research use only. Not for food, drug, household, or cosmetic use.
Availability:
2-3 Weeks
Shipping:
$14.99 Ground shipping (In continental US only.)

    Description

    Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

    The maximum flow rate at 15 cm bed height is >1000 cm/h and the maximum pressure is >300 kPa. The binding capacity with an approximately 30 kDA protein is 5 mg/mL.

    These products are excellent for large scale His-tagged protein purification:

    • Easy scale up and robust function
    • High chemical and physical stability
    • Good resolution in minimum time

    HTC beads are high throughput beads designed to meet the demand for industrial process separation, suitable for use with FPLC or HPLC, and can withstand pressures up to 300 kPa. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

    Storage/Handling: Store at 4°C. Do not freeze.

    Product Specifications

    Catalog ID: R-203
    Storage/handling: Store at 4°C. Do NOT freeze.
    UN number: NA-1993
    group number: III
    Binding Loading Capacity: ~15 (μmol Me2+/ml gel)
    Chemical Stability: Stable in all commonly used reagents.
    Maximum Flow Rate: 1000 cm/h at 15 cm bed height
    Maxium Pressure: 300 kPa at 15 cm bed height
    Temperature Stability: Autoclavable at 120°C for 20 min in H2O
    Storage Buffer: 20% Ethanol
    Cross-linking: Highly crosslinked
    Bead Size: Spherical, Standard: ~50-150 μm (approx.)

    CITATIONS

    Featured Articles

    • Why Did My Nickel Agarose Beads Lose Their Color?

      Why Did My Nickel Agarose Beads Lose Their Color?

      Nickel agarose beads have a beautiful blue color. This blue color isn’t just for aesthetics; it is also a useful tracker of your purification or...

      Read more

    • Why are nickel agarose beads blue - thumbnail

      Why Are Nickel Agarose Beads Blue?

      Many experimental procedures in science have no way to monitor in real time how the procedure is going. Think about it, we’re usually pipetting colorless...

      Read more

    • IDA vs. Nickel Beads thumbnail and banner image

      What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?

      There are a lot of options when choosing nickel agarose beads for purifying your his-tagged proteins. So, the decision on which beads to use can...

      Read more

    • columns and agarose beads article thumbnail

      Conjugating Ligands to Agarose Beads for Affinity Purification

      Affinity purification is a frequently used technique for purifying proteins. During affinity purification, affinity tags or intrinsic properties of the proteins are used to bind...

      Read more

    • How agarose beads are made thumbnail image

      How Agarose Beads are Made

      Agarose beads are a foundational tool used for various protein purification techniques. Most frequently, agarose beads are purchased through a vendor such as GoldBio. However,...

      Read more

    Login

    Forgot your password?

    Don't have an account yet?
    Create account