Coelenterazine 400a

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Description

Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.

Product Specifications:
Coelenterazine 400a
2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one
DeepBlueCTM

Formula: C26H21N3O

MW: 391.48 g/mol

Purity: >95% by TLC

Color: Yellow to orange powder

Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
Soluble in methanol or ethanol (NOT in DMSO).
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

PubChem CID: 135750016

Coelenterazine 400a

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Catalog Number:
C-320-1
CAS Number:
70217-82-2
$222.00

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    Description

    Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.

    Product Specifications:
    Coelenterazine 400a
    2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one
    DeepBlueCTM

    Formula: C26H21N3O

    MW: 391.48 g/mol

    Purity: >95% by TLC

    Color: Yellow to orange powder

    Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
    Soluble in methanol or ethanol (NOT in DMSO).
    Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

    PubChem CID: 135750016

    Product Specifications

    Catalog ID: C-320
    CAS #: 70217-82-2
    MW: 391.48 g/mol
    Grade: MOLECULAR BIOLOGY GRADE
    Storage/handling: Store at -20°C. Protect from light.

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