Description
Cy3‐NHS Ester is an amine-reactive Cy3 labeling dye that carries only one reactive group on each dye molecule for accurate labeling of amine groups on antibodies or oligonucleotides.
Cy3 is a bright, photostable, and pH insensitive fluorescent dye used to label antibody, proteins, peptides. Cy3 conjugates give less background than TAMRA and most other fluorophores.
Cy3 dyes are water-soluble labeling reagent. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional TAMRA (tetramethylrhodamine) filter sets since the excitation and emission spectra are nearly identical to those of TAMRA.
Formula: C36H44N3O13S3
Storage/Handling: Store at -20°C. Product shipped at ambient temperature.
Soluble in Water, DMSO or DMF.
Optical Properties:
| λabs | 550 nm |
| λem | 570 nm |
| Extinction Coefficient | 150,000 M-1 cm-1 |
| CF280 | 0.08 |
| CF260 | 0.08 nm |
| Molecular Weight | 1024.94 g/mol |
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