Description
GoldBio’s D-Luciferin Sodium Salt, Endotoxin Free is a high-purity bioluminescent substrate engineered for sensitive in vivo and cell-based luciferase assays.
This endotoxin-free formulation eliminates lipopolysaccharide contamination, preventing unwanted immune activation and ensuring accurate, reproducible results in applications such as tumor imaging, gene expression analysis, and stem cell tracking.
With excellent solubility and strong signal output, the sodium salt form is ideal for preparation in physiological buffers like PBS.
Tested and Certified for in vivo imaging (See "Luciferin FAQ" in additional information)
Research Applications for Endotoxin-Free D-Luciferin Sodium Salt
(Click each for more information)
In Vivo Bioluminescent Imaging in Endotoxin-Sensitive Animal Models
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Purpose: To monitor biological processes in live animals without immune interference from endotoxin contamination.
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How It Works: D-Luciferin is oxidized by firefly luciferase in the presence of ATP, producing light (~560 nm). The endotoxin-free formulation ensures no activation of innate immune responses that could alter imaging results.
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Applications: Tumor progression studies, infection models, inflammation research, longitudinal imaging.
Contag, C. H., & Bachmann, M. H. (2002). Advances in in vivo bioluminescence imaging. Annual Review of Biomedical Engineering, 4, 235–260.
Luciferase Reporter Assays in Mammalian Cells
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Purpose: To quantify gene expression and signaling pathways without introducing cellular stress artifacts.
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How It Works: Luciferase-expressing cells emit light upon addition of D-Luciferin; endotoxin-free conditions ensure accurate signal measurement and cell viability.
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Applications: Promoter assays, pathway activation studies, drug screening.
Brasier, A. R., & Ron, D. (1992). Luciferase reporter gene assay in mammalian cells. Methods in Enzymology, 216, 386–397.
Stem Cell and Cell Therapy Tracking
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Purpose: To monitor transplanted or engineered cells without introducing inflammatory artifacts.
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How It Works: Only viable luciferase-expressing cells generate signal; endotoxin-free luciferin ensures accurate readouts of cell survival and localization.
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Applications: Stem cell engraftment, CAR-T tracking, regenerative medicine studies.
Cao, F., et al. (2006). In vivo visualization of embryonic stem cell survival, proliferation, and migration. Circulation, 113(7), 1005–1014.
High-Precision Bioluminescent Quantification in Drug Screening
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Purpose: To ensure accurate signal measurement in assays where background immune activation could skew results.
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How It Works: Eliminating endotoxin reduces variability and improves reproducibility of luciferase-based readouts.
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Applications: High-throughput screening, toxicity assays, pathway modulation studies.
Fan, F., & Wood, K. V. (2007). Bioluminescent assays for high-throughput screening. Assay and Drug Development Technologies, 5(1), 127–136.
Recommended Applications
· In vivo bioluminescent imaging (IVIS or similar systems)
· Preparation of D-Luciferin in PBS for injection
· Luciferase reporter assays in mammalian cells
· Immune and inflammation-sensitive studies
· High-throughput bioluminescent screening
Benefits
· Eliminates endotoxin-related variability
Prevents immune activation that can confound in vivo and cell-based experiments.
· Optimized for sensitive biological systems
Ideal for immunology, stem cell, and therapeutic studies.
· High reproducibility in bioluminescent assays
Reduces background noise and improves data consistency.
· Excellent solubility and bioavailability
Sodium salt form supports efficient delivery in vivo, with solubility up to 100 mg/ml in aqueous buffers.
· Trusted for critical experiments
Designed for applications where purity directly impacts experimental outcomes.
Functional Highlights and Mechanism
· Endotoxin-Free Advantage
Removes lipopolysaccharide (LPS) contamination that can activate immune signaling pathways.
· Luciferase-Driven Light Production
D-Luciferin is oxidized by firefly luciferase in the presence of ATP and oxygen, producing measurable light.
· Physiological Compatibility
Works effectively in PBS or other isotonic buffers for in vivo administration.
Recommended Applications
· Dissolve in sterile PBS for in vivo applications.
· Filter sterilize before injection if required.
· Prepare fresh solutions or aliquot and store at –20°C.
· Protect from light to prevent degradation.
Storage/Handling
Store desiccated at -20°C. Protect from light.
