Description
Enhanced Performance | Greater Yields | Cost-Effective
Dr. Gold’s Bacterial Protein Extraction Reagent is a powerful, next-generation solution designed for efficient extraction of expressed proteins from bacterial cells—now reengineered for improved performance and cost-effectiveness. Built on the foundation of our trusted Bacterial Cell Lysis Buffer, this ready-to-use formulation offers enhanced yield and reproducibility without the need for harsh mechanical disruption.
Our proprietary blend of buffering agents and dialyzable, non-denaturing detergents ensures gentle but thorough lysis, preserving native protein structure and activity.
The reagent includes lysozyme, nucleases, and a specially optimized salt solution to promote full membrane disruption and maximize the recovery of soluble proteins.
Whether you're preparing samples for SDS-PAGE, purification, or enzymatic assays, Dr. Gold’s reagent offers superior protein extraction with consistent, repeatable results—batch after batch.
Key Features
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Ready-to-use formulation—no additional components required
- Contains both non-ionic and zwitterionic detergents in Tris buffer
- Includes lysozyme and nucleases for complete cell breakdown
- Enhanced salt formulation improves soluble protein recovery
- Compatible with common reducing agents and protease inhibitors
- Suitable for use with both fresh and frozen bacterial pellets
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Outperforms leading competitors in soluble protein yield and clarity
Kit Component
- Dr. Gold’s Bacterial Protein Extraction Reagent
- GB Enzyme Mix
- Salt Solution
Note: The Salt Solution is not strictly required for lysis, but our research has shown it significantly enhances protein solubility and yield.
Storage/Handling
This product may be shipped with blue ice. Dr. Gold’s Bacterial Protein Extraction Reagent and Salt Solution should be stored at 4°C, GB Enzyme Mix should be stored at -20°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
FAQ
How much reagent do I need to use with my culture?
Use 1.3 mL of extraction buffer per 10 mL of culture at A600 = 2.0. Adjust proportionally for different cell densities and volumes.
Does the reagent contain EDTA or other chelating agents? is the reagent compatible with metal affinity chromatography (His tagged proteins)?
No! Dr. Gold’s Bacterial Protein Extraction Reagent does not contain EDTA or other chelating agents. It is fully compatible with His-Tag affinity chromatography.
Can proteins isolated using Dr. Gold’s Bacterial Protein Extraction Reagent be quantified using Bradford or BCA or will there be background due to lysozyme and nucleases?
There may be some background signal from the added enzymes, but in our testing it's not enough to skew the total extraction results (Levels are undetectable on SDS-Page Gels of extracted material).
