Description
Fold-Assist Chemically Competent E. Coli Cell Bundle
Designed to support the expression of challenging recombinant proteins, the Fold-Assist™ Chaperone Competent Cell Bundle features E. coli BL21 (DE3) strains engineered to promote proper folding through co-expression of specialized molecular chaperone systems.
This bundle includes five strains, each carrying a distinct Fold-Assist™ chaperone plasmid (pFA series), designed to provide complementary folding environments for difficult targets. These systems correspond to widely used chaperone configurations and include combinations of DnaK/DnaJ/GrpE (KJE), GroEL/ES, and Trigger Factor:
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pFA-KJE-Gro — combined DnaK/DnaJ/GrpE + GroEL/ES system
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pFA-KJE — DnaK/DnaJ/GrpE system
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pFA-Gro — GroEL/ES system
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pFA-GroTF — GroEL/ES + Trigger Factor system
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pFA-TF — Trigger Factor system
Each plasmid has been engineered in a stabilized, low-burden format to support consistent maintenance and reliable co-expression alongside high-level protein production systems.
Built on the trusted BL21 (DE3) background, these strains enable robust T7-driven expression while minimizing proteolysis, making them ideal for producing soluble, functional protein.
Key Features:
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Five Fold-Assist™ chaperone systems: Screen multiple folding pathways to identify optimal expression conditions
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Functionally aligned with industry-standard systems: Corresponding to common KJE, GroEL/ES, and Trigger Factor-based chaperone configurations
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Enhanced protein solubility: Reduce inclusion body formation and increase recovery of active protein
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Optimized plasmid design: Engineered for stable maintenance and reduced cellular burden during co-expression
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Compatible with standard expression vectors: Designed to perform alongside common high-expression plasmid systems
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Inducible chaperone expression: Fine-tune folding support independently of target protein production
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Free of animal derived components and grown with animal free media.
Applications:
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Expression of insoluble or aggregation-prone proteins
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Production of eukaryotic or multi-domain proteins in E. coli
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Structural biology and protein characterization
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Optimization of recombinant protein yield and activity
Workflow Advantage:
Each strain is pre-transformed with a chaperone plasmid, eliminating the need for sequential cloning and strain construction. This enables rapid screening of multiple folding environments using a single transformation step
Kit Components:
- 2x 100µL of each Chaperone Competent E. Coli Cell line (10x tubes in total)
- 2 x 12mL Recovery Media
- 1 x 10µL Control Plasmid (pUC19 Control, 500 pg/µL)
Storage/Handling:
This product is shipped on dry ice. Chemically Competent E. coli cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
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