Description
GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen™ is far more sensitive than SYBR Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen™ is very stable, both hydrolytically and thermally. GelGreen™ is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader®).
GelGreen™ can be used for either post-electrophoresis gel staining or precast gel staining.
A series of safety tests have confirmed that GelGreen™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelGreen™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.
GelGreen™ 10,000X solution in DMSO is for established users of GelGreen™ in DMSO who do not wish to change their laboratory protocols. For new users, we recommend GelGreen™ 10,000X in water (catalog # G-745), which eliminates the hazards of handling DMSO for even better safety. The performance and stability of GelGreen™ 10,000X is comparable in both the water and DMSO formulations.
Features
- Safer than Ethidum Bromide
| Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
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| | Passed environmental safety tests for direct disposal down the drain or in regular trash
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| | Higher sensitivity than EtBr and SYBR® Safe
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| | Available in water, stable at room temperature for long-term storage and microwavable
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| | Very simple procedures for precast or post-electrophoresis gel staining
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- Compatible with a standard UV transilluminator or a gel reader with visible light excitation
| Replaces SYBR® or GelStar® with no optical setting change
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- Compatible with downstream applications
| Compatible with gel purification, restriction digest, sequencing and cloning
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Storage/Handling: Store at room temperature for up to 2 years. Protect from light.
GelGreen and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.
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