Glutathione Agarose Resin

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Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

This product is supplied as a 75% (v/v) aqueous suspension in 20% EtOH.

Fusion proteins expressed from pGEX vectors contain a Glutathione S-transferase (GST) moiety and can therefore be purified to near homogeneity by affinity chromatography on glutathione as a substrate in inactivate toxic small molecules via formation of mercapturic acid. Because the affinity of GST for its substrate is in the submillimolar range, immobilization of glutathione on an agarose matrix makes a highly efficient affinity chromatography resin.

This product provides a one-step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Bound GST –fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione.


Table 1: Glutathione Working Conditions


Volumetric flow rate (mL / min)

Column diameter
(mm)

Bed volume
(mL)

Packing

Equilibration/Washing/Elution

Binding

6.6

1.0

1.4

1.0

0.3 - 1.0

16

10

7.0

5.0

0.5 - 5.0








Linear flow rate (cm/h)



<250

<180

<180


Storage/Handling

Store at 4°C. Do not freeze.

Glutathione Agarose Resin

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Catalog Number:
G-250-5
CAS Number:
$109.00

Availability:
In Stock
Shipping:
Shipping calculated at checkout

    Description

    Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

    This product is supplied as a 75% (v/v) aqueous suspension in 20% EtOH.

    Fusion proteins expressed from pGEX vectors contain a Glutathione S-transferase (GST) moiety and can therefore be purified to near homogeneity by affinity chromatography on glutathione as a substrate in inactivate toxic small molecules via formation of mercapturic acid. Because the affinity of GST for its substrate is in the submillimolar range, immobilization of glutathione on an agarose matrix makes a highly efficient affinity chromatography resin.

    This product provides a one-step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Bound GST –fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione.


    Table 1: Glutathione Working Conditions


    Volumetric flow rate (mL / min)

    Column diameter
    (mm)

    Bed volume
    (mL)

    Packing

    Equilibration/Washing/Elution

    Binding

    6.6

    1.0

    1.4

    1.0

    0.3 - 1.0

    16

    10

    7.0

    5.0

    0.5 - 5.0








    Linear flow rate (cm/h)



    <250

    <180

    <180


    Storage/Handling

    Store at 4°C. Do not freeze.

    Product Specifications

    Catalog ID: G-250
    Storage/handling: Store at 4°C. Do NOT freeze.

    CITATIONS

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